摘要
为进一步探究胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)感染仔猪的致病机制,利用SYBR Green荧光定量PCR方法,探究仔猪感染后IL-1β、IL-6、IL-8及caspase-3基因表达水平的变化。通过肺灌洗液获得猪肺巨噬细胞,将APP以MOI=10的剂量感染细胞,并对经APP感染后的肺巨噬细胞相关细胞因子(IL-1β、IL-6、IL-8及caspase-3)基因表达水平进行检测。结果显示,APP感染后3~12 h,IL-1β、IL-6、IL-8及caspase-3基因表达量呈升高趋势,感染后12~24 h达到高峰。结果提示,APP感染早期IL-1β、IL-6、IL-8等细胞因子在宿主清除致病菌过程中发挥着重要作用。本研究为下一步深入探索APP致病机制奠定了基础。
In order to further investigate the pathogenic mechanism of Actinobacillus pleuropneumoniae(APP)infection in piglets,the changes in the expression levels of IL-1β,IL-6,IL-8 and caspase-3 genes in infected piglets were investigated by SYBR green fluorescence quantitative PCR assay.Porcine alveolar macrophages(PAMs)were obtained through bronchoalveolar lavage fluid and infected by APP at a dose of MOI=10,then the expression levels of relevant cytokine genes(IL-1β,IL-6,IL-8 and caspase-3)were detected.The results showed that the amounts of gene expression of IL-1β,IL-6,IL-8 and caspase-3 were increasing at 3~12 h after APP infection,and reached a peak at 12~24 h.In conclusion,IL-1β,IL-6 and IL-8 played an important role in clearing pathogenic bacteria by hosts at early stage of APP infection.A foundation was laid for further exploration of APP's pathogenic mechanism.
作者
刘红洋
邵清
邴新帅
王秋东
巩俊明
朱奕霏
王羽辉
田喜荣
金哲
姜艳雪
Liu Hongyang;Shao Qing;Bing Xinshuai;Wang Qiudong;Gong Junming;Zhu Yifei;Wang Yuhui;Tian Xirong;Jin Zhe;Jiang Yanxue(Shuangliao Center for Animal Disease Prevention and Control,Shuangliao,Jilin 136400,China;Shuangliao Service Center for Livestock and Poultry Slaughter,Shuangliao,Jilin 136400,China;Shuangliao Animal Husbandry Service Station,Shuangliao,Jilin 136400,China;Jilin Center for Animal Disease Prevention and Control,Changchun,Jilin 130000,China;Shuangliao Animal Quarantine Station,Shuangliao,Jilin 136400,China)
出处
《中国动物检疫》
CAS
2023年第12期98-103,共6页
China Animal Health Inspection
