摘要
目的基于白细胞介素-6(IL-6)/STAT3通路研究苗药方剂“四大血”(SX)对胶原诱导性关节炎(CIA)小鼠的保护作用机制。方法取40只C57BL/6雄性小鼠随机均分为4组[正常组、模型组、SX组和甲氨蝶呤(MTX)组],采用鸡Ⅱ型胶原和完全弗氏佐剂混合乳化剂诱导类风湿性关节炎(RA)小鼠模型。造模成功后按照实验分组(每组6只)进行灌胃给药。分别检测各组小鼠的足肿胀程度、关节病理改变、关节炎指数(AI)评分、肿瘤坏死因子-α(TNF-α)和IL-6水平、关节滑膜组织中p-STAT3蛋白表达情况。结果SX对RA疾病相关指标有显著改善作用,可以降低小鼠足肿胀程度、关节炎滑膜炎症、AI评分、TNF-α和IL-6水平,抑制p-STAT3蛋白的表达。结论SX可以发挥明显的抗RA作用,其可通过抑制IL-6/STAT3信号通路蛋白(p-STAT3)表达发挥对CIA小鼠的保护作用。
Objective To study the protective effect and mechanism of the Miao ethnomedicine recipe"Sidaxue"(SX)on collagen induced arthritis(CIA)mice based on the interleukin-6(IL-6)/STAT3 pathway.Methods Forty male C57BL/6 mice were randomly divided into 4 groups[normal group,model group,SX group and methotrexate(MTX)group],and the rheumatoid arthritis(RA)mouse model was induced by combination of chicken typeⅡcollagen and complete Freund's adjuvant.After successful modeling,the drug was administered by gavage according to experimental groups(6 mice in each group).The degree of foot swelling,joint pathological changes,Arthritis Index(AI)score,tumor necrosis factor-α(TNF-α)and IL-6 levels,and p-STAT3 protein expression in joint synovium were detected respectively.Results SX can significantly improve RA disease-related indicators,reduce the degree of foot swelling,arthritis synovitis,AI score,TNF-αand IL-6 levels,and inhibit p-STAT3 protein expression.Conclusion SX can exert a significant anti-RA effect and exert a protective effect on mice with CIA by inhibiting the expression of IL-6/STAT3 signaling pathway protein(p-STAT3).
作者
谢佳
罗进芳
XIE Jia;LUO Jinfang(Guizhou University of Traditional Chinese Medicine,Guiyang 550025;Key Laboratory on the Property&Effect of Chinese Medicine(Ethnic Medicine),Guizhou Province,Guiyang 550025,China)
出处
《临床医学研究与实践》
2024年第1期1-5,共5页
Clinical Research and Practice
基金
贵州省科技计划项目(自然科学)(黔科合基础-ZK[2022]一般477)
2022年度贵州省卫生健康委科学技术基金项目(No.gzwkj2022-055)
贵州省中医药管理局中医药、民族医药科学技术研究课题(No.QZYY-2021-012)
贵州中医药大学大学生创新创业训练计划项目[贵中医大创合字(2021)8号]。
关键词
苗药方剂
四大血
类风湿性关节炎
胶原诱导性关节炎
Miao ethnomedicine recipe
Sidaxue
rheumatoid arthritis
collagen induced arthritis
