摘要
【目的】明确鹰嘴桃果实组织海绵化的分子机制。【方法】对鹰嘴桃病害果海绵组织、非病害组织和健康果实组织进行转录组测序。【结果】在病害果海绵组织vs健康果实组织、非病害组织vs健康果实组织、病害果海绵组织vs非病害组织的转录组比较中,分别鉴定到4557、4446、672个差异表达基因。病害果海绵组织与健康果的差异表达基因主要富集在新陈代谢、碳水化合物代谢、能量代谢、光合作用等通路。与健康果或病害果非病变组织相比,鉴定出12个与细胞壁代谢相关的差异表达基因(PG-At1g48100、PG-QRT3、PG、6个XET2、BXL7、2个EXP-A4)在鹰嘴桃病害果海绵组织表达上调;此外,3个钙转运基因(ACA13)和2个钙传感器基因(CaM11、CML18)在鹰嘴桃病害果海绵组织表达上调。其他钙传感器相关基因的表达水平在病害果中出现不同程度的上调和下调。【结论】鉴定出12个与细胞壁代谢、3个与钙转运和23个与钙传感器相关的差异表达基因,推测钙代谢以及细胞壁代谢异常在果实组织海绵化过程中发挥关键作用。
【Objective】Spongy tissue is a serious physiological disorder in Olecranon peach(Prunus persica L.).The symptom occurs about 10 days before fruit ripening,and the pulp becomes spongy in texture and brown in colour,causing significant economic losses in peach production.However,little has been known about the underlying mechanism causing spongy tissue up to now.Here,the compara-tive transcriptomics was used to explore the molecular mechanism of spongy tissue formation.【Meth-ods】Samples from spongy tissue(BGHM)and non-spongy tissue(BGFB)in unhealthy flesh,and tis-sue in healthy fruit flesh(JKG)of Olecranon peach were collected and used for total RNA extraction.The high-throughput sequencing(HTS)data of transcriptome was generated with HiSeq 6000 platform.The published genome of P.persica(GenBank accession:GCF_000346465.2)was used as a reference.The HTS reads were mapped to the reference genome and the expression level of each transcript was de-termined by calculating transcript per million(TPM)with FADU.Differentially-expressed genes(DEGs)were identified using DESeq with the screening criteria of p<0.01 and|log2FC|>1.0.For func-tional analyses,GO and KEGG enrichment analyses were performed to investigate the major pathways of DEGs.【Results】Clean reads per sample generated by RNA-seq ranged from 18.4 to 30.5 million reads,and the mapping rate ranged from 95.08%to 95.67%.A total of 4557 DEGs were identified be-tween spongy tissue and healthy fruit flesh(BGHM vs JKG),2410 genes were up-regulated and 2127 genes were down-regulated.672 DEGs were identified between spongy tissue and non-spongy tissue(BGHM vs BGFB),including 539 up-regulated genes and 133 down-regulated genes.4446 DEGs were identified between non-spongy tissue and healthy fruit flesh(BGFB vs JKG),with 2121 up-regulated and 2323 down-regulated genes.The GO terms enriched for DEGs of BGHM vs JKG were 190.In mo-lecular function,ion binding,oxidoreductase activity,and inorganic molecular entity transmembrane transporter activity were significantly
作者
卢锦明
林心悦
廖永林
LU Jinming;LIN Xinyue;LIAO Yonglin(Institute of Plant Protection,Guangdong Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory High Technology for Plant Protection,Guangzhou 510640,Guangdong,China;College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Guangdong Yueke Plant Protection Agricultural Technology Co.,Ltd,Guangzhou 510640,Guangdong,China)
出处
《果树学报》
CAS
CSCD
北大核心
2023年第12期2524-2535,共12页
Journal of Fruit Science
基金
连平县鹰嘴蜜桃产业园专家工作站(2021工作站09)
2021年广东省农村科技特派员驻镇帮镇扶村项目(KTP20210015)。
关键词
鹰嘴桃
海绵组织
生理性病害
转录组
基因分析
Olecranon peach
Spongy tissue
Physiological disorder
Transcriptome
Gene analysis
