摘要
目的:基于网络药理学探讨苍术-黄柏药对治疗糖尿病肾病(diabetic kidney disease,DKD)的有效成分、潜在作用靶点及作用机制。方法:通过中药系统药理学平台检索苍术-黄柏药对的有效成分、潜在作用靶点,在人类基因数据库和人类孟德尔遗传数据库数据库筛选与糖尿病肾病有关的疾病靶点,然后绘制Venn图,得出苍术-黄柏药对治疗DKD的共同靶点,借助Cytoscape 3.7.2构建“药对成分-靶点-疾病”可视化网络。利用STRING平台绘制交集靶点PPI网络,并利用MCODE插件筛选核心子网络,在David数据库针对核心靶点进行GO及KEGG通路富集分析。结果:检索筛选出苍术-黄柏药对有效活性成分28个,潜在作用靶点202个,其中,苍术-黄柏药对治疗DKD的133个,通过PPI核心子网络发现包括JUN、MMP9、CXCL8、TNF、VEGFA、PTGS2、AKT1、IL-6等在内的52个靶点可能是药对治疗DKD的核心靶点,KEGG通路富集分析发现苍术-黄柏药对治疗DKD的作用机制可能与磷脂酰肌醇3激酶-蛋白激酶B(phosphatidylinositol 3 kinase-protein kinase B,PI3K-Akt)、肿瘤坏死因子(tumor necrosis factor,TNF)、Toll样受体(toll-like receptor,TLR)及缺氧诱导因子1(hypoxia inducible factor-1,HIF-1)等88条信号通路有关。结论:苍术-黄柏药对治疗DKD体现了多成分、多靶点、多途径的特点,为进一步探讨其作用机制提供了参考。
Objective:To study the effective components,potential targets and mechanism of Cangzhu(Atractylodis rhizoma)-Huangbo(Phellodendri Chinensis cortex)in the treatment of diabetic kidney disease(DKD)based on network pharmacology.Methods:The active ingredients and potential targets of Cangzhu-Huangbo were searched from TCMSP,the targets related to DKD were screened in the Genecards and OMIM databases,and the common targets of the drug pair for DKD were obtained by drawing Venn diagrams.The drug pair components-target-disease visualization network was constructed via Cytoscape 3.7.2.STRING platform was utilized to construct PPI network of the intersection targets,MCODE was applied to screen the core subnetwork,GO and KEGG pathway enrichment analysis of the core targets were conducted in David database.Results:All 28 active ingredients and 202 potential targets were identified from the drug pair Cangzhu-Huangbo,among them,there were 133 targets belonging to the drug pair in the treatment of DKD,52 targets including JUN,MMP9,CXCL8,TNF,VEGFA,PTGS2,AKT1,IL-6 found by PPI core subnetwork might be the core ones of the drug pair in the treatment of DKD,KEGG pathway enrichment analysis indicated that the mechanism of the drug pair in treating DKD might be related to 88 signaling pathways including PI3K-Akt,TNF,TLR and HIF-1.Conclusion:The couplet medicine Cangzhu-Huangbo in the treatment of DKD reflect the characteristics of many ingredients,mangy targets and many pathways,and it could provide the reference for further discussion of its mechanism.
作者
陈莉
安海燕
张承承
郭晓媛
CHEN Li;AN Haiyan;ZHANG Chengcheng;GUO Xiaoyuan(Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078,China)
出处
《西部中医药》
2023年第12期77-82,共6页
Western Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81904144)。
关键词
糖尿病肾病
网络药理学
苍术
黄柏
作用机制
diabetic kidney disease
network pharmacology
Cangzhu
Huangbo
the mechanism
