摘要
目的 :探究散结片对肝癌大鼠巨噬细胞极化及MAPK/JNK信号通路的作用机制。方法 :选取SPF级SD雄性裸鼠,随机分为正常组、模型组、索拉非尼组、散结片组,记录大鼠死亡数量并绘制生存曲线,H-E染色检测肝组织病理形态,免疫组织化学显色检测肝组织巨噬细细胞特异型标志物的蛋白表达,免疫印迹法检测肝组织MAPK/JNK信号通路相关蛋白表达。结果 :与正常组比较,模型组大鼠死亡数量、肝组织CD163、p38MAPK、JNK蛋白表达明显增多,生存曲线、肝组织中CD11c蛋白表达明显降低;与模型组比较,索拉非尼组、散结片组死亡数量、肿瘤质量及体积、肝组织CD163、p38MAPK、JNK蛋白表达显著减少,生存曲线、肝组织中CD11c蛋白表达显著升高,且散结片组比索拉非尼组变化显著;而抑制剂组与散结片组肝织中p38MAPK、JNK蛋白表达相比差异无统计学意义,联合组比抑制剂组明显降低。结论 :散结片可能是通过抑制MAPK/JNK信号通路,抑制巨噬细胞向M2型极化,促进其向M1型极化,进而抑制肝癌大鼠肿瘤生长。
Objective:To explore the effect mechanism of Sanjie tablet on macrophage polarization and MAPK/JNK signaling pathway in rats with liver cancer.Methods:SPF grade male nude mice(SD strain)were randomly divided into normal group,model group,sorafenib group,and Sanjie tablet group.The number of deaths of the rats was recorded,and survival curves were plotted.HE staining was used to examine the pathological morphology of liver tissues,immunohistochemistry was used to detect the protein expression of macrophage-specific markers in liver tissues,and immunoblotting was used to detect the protein expression of MAPK/JNK signaling pathway-related proteins in liver tissues.Results:Compared with the normal group,the number of deaths,CD163,p38MAPK,and JNK protein expression in liver tissues of the model group were significantly increased,the survival curve,and CD11c protein expression in liver tissues were significantly decreased.Compared with the model group,the sorafenib group and Sanjie tablet group showed significantly reduced number of deaths,tumor mass and volume,CD163,p38MAPK,and JNK protein expression in liver tissues,and significantly increased survival curve and CD11c protein expression in liver tissues.Additionally,the Sanjie tablet group showed significant differences compared with the sorafenib group.There was no statistically significant difference in p38MAPK and JNK protein expression in liver tissues between the inhibitor group and Sanjie tablet group,while the combined group showed a significant decrease compared with the inhibitor group.Conclusion:Sanjie tablet may inhibit the tumor growth of rats with hepatocellular carcinoma by suppressing MAPK/JNK signaling pathway,inhibiting the polarization of macrophages towards M2 type and promoting the polarization towards M1 type.
作者
张远
董晶
国滨
徐振华
时桢
李金芳
无
Zhang Yuan;Dong Jing;Guo Bin;Xu Zhenhua;Shi Zhen;Li Jinfang;无(Jinshazhou Hospital,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510080;Department of Radiation Oncology,Shouguang People's Hospital,Shouguang 262700;Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China)
出处
《解剖学杂志》
CAS
2023年第5期406-410,439,共6页
Chinese Journal of Anatomy
基金
国家自然科学基金培育项目(19NSP012)。
