摘要
【目的】探究四川烤烟游离氨基酸与烟叶感官和外观品质的关联性,确定影响烟叶品质的关键氨基酸指标,为四川优质烟叶的定向调控及品质提升提供理论参考。【方法】采集四川省5个烤烟主产区(凉山、攀枝花、宜宾、泸州和广元)188份烟叶样品,进行外观质量和感官质量评价,测定游离氨基酸含量,采用Pearson相关分析及曼特尔(Mantel)检验系统分析游离氨基酸含量和各类氨基酸占比与感官、外观质量等品质指标的相互关系,筛选影响四川烟叶品质的关键氨基酸指标,并分析5个产区关键氨基酸指标的含量分布特征。【结果】四川烤烟共检出17种游离氨基酸,总量均值为14.2 mg/g,其中脯氨酸含量最高,占氨基酸总量的83.4%,异亮氨酸含量最低,均值仅为7.6μg/g;17种游离氨基酸含量表现为上部叶>中部叶,且部位间差异均达极显著水平(P<0.01,下同)。相关分析结果表明,17种游离氨基酸指标间主要为正相关,异亮氨酸、苯丙氨酸、缬氨酸、苏氨酸、组氨酸、谷氨酰胺、亮氨酸、碱性氨基酸占比、芳香族氨基酸占比、含羟基类氨基酸占比和酰胺类氨基酸占比与烟叶品质呈负相关,亚氨基酸占比与烟叶品质呈正相关;Mantel检验进一步揭示碱性氨基酸占比、组氨酸、苯丙氨酸和缬氨酸是影响四川烤烟品质的关键氨基酸指标。四川省5个烤烟主产区中凉山烟叶的关键氨基酸指标含量大多处于中等或偏低水平,广元烟叶的大多数关键氨基酸指标含量偏高。【结论】四川初烤烟叶游离氨基酸指标间均存在中等程度或高度相关性,大部分游离氨基酸指标与烟叶品质呈负相关。生产中适当控制游离氨基酸含量,提高亚氨基酸占比,有利于四川烤烟品质提升。
【Objective】The purpose of this study was to explore the correlation between free amino acids and sensory and appearance quality of flue-cured tobacco,and to identify the key amino acid indexes affecting the quality of tobacco,so as to provide theoretical references for the directional regulation and quality enhancement of high-quality tobacco in Sichuan.【Method】A total of 188 tobacco samples were collected from five main flue-cured tobacco producing areas in Sichuan(Liangshan,Panzhihua,Yibin,Luzhou and Guangyuan),and the appearance quality and sensory quality were evaluated,as well as the content of free amino acids.Pearson correlation analysis and Mantel test were used to analyze the relationship between the content of free amino acids,the proportion of various amino acids and the sensory and appearance quality indexes,identify the key amino acid indexes affecting the quality of Sichuan tobacco and analyze the distribution characteristics of the key amino acid indexes in the five production areas.【Result】Atotal of 17 free amino acids were detected in Sichuan flue-cured tobacco,with an average total content of 14.2 mg/g.Among them,the content of proline was the highest,accounting for 83.4%of the total amino acid,and the content of isoleucine was the lowest,with an average of only 7.6μg/g.The contents of the 17 free amino acids were showed as upper leaves>the middle leaves,and the differences between the stalk positions reached the extremely significant level(P<0.01,the same below).Correlation analysis showed that there were mainly positive correlations among 17 free amino acids.Isoleucine,phenylalanine,valine,threonine,histidine,glutamine,leucine,the proportion of alkaline amino acids,the proportion of aromatic amino acids,the proportion of hydroxyl amino acids,and the proportion of amide amino acids were negatively correlated with tobacco quality,while the proportion of imino acids was positively correlated with tobacco quality.Mantel test further revealed that the proportion of alkaline amino acids
作者
韶济民
张海燕
李奇
别瑞
庄志麟
邱军
靳冬梅
曹建敏
SHAO Ji-min;ZHANG Hai-yan;LI Qi;BIE Rui;ZHUANG Zhi-lin;QIU Jun;JIN Dong-mei;CAO Jian-min(Sichuan Tobacco Quality Supervision and Testing Station,Chengdu,Sichuan 610041,China;Technology Center,China Tobacco Zhejiang Industrial Co.,Ltd.,Hangzhou,Zhejiang 310024,China;Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao,Shandong 266101,China;Graduate School,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Hongta Liaoning Tobacco Co.,Ltd.,Shenyang,Liaoning 110003,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2023年第8期2279-2288,共10页
Journal of Southern Agriculture
基金
中国农业科学院科技创新工程项目(ASTIP-TRIC07)
中国烟草总公司四川省公司科技项目(SCYC202118)。
