摘要
异戊二烯是一种重要的工业基础原料,利用生物法合成异戊二烯以其经济性和可持续性受到了大量研究和关注,但高昂的底物成本阻碍了其生产规模的进一步扩大。提出了以厌氧消化污泥(ADS)为唯一底物,通过基因工程菌生物合成异戊二烯的方法,实现了对ADS中有机物的去除和高价值产品的回收。污泥为唯一底物生产异戊二烯的最高产量(以底物的COD为基准)可达40.5 mg/g,污泥的生物毒性、pH及生物可利用碳源的含量影响着异戊二烯产率。通过优化基因工程菌代谢通路设计,可提升其抗污染负荷能力和底物转化效率,从而进一步提高异戊二烯产率,为废弃厌氧消化污泥中有机物资源化的回收利用和高价值异戊二烯合成提供了参考。
Isoprene is an important bulk basic raw material,and its production by biosynthesis has received a lot of research and attention in recent years because of its economy and sustainability.However,the relatively expensive substrate addition obstructed further expansion of production.Biosynthesis of isoprene by genetically engineered bacteria using anaerobic digestion sludge(ADS)as the sole substrate was proposed to achieve the removal of organic matter and recovery of high-value products in ADS.Taking the waste sludeg as the sole carbon source could produce 40.5 mg/g isoprene(the substrate COD as basis).The bio-toxicity,pH and bioavailable carbon source of ADS affected isoprene production.By optimizing the metabolic pathway design of genetically engineered bacteria,its antipollution loading capacity and substrate conversion efficiency could be improved,so as to further improve the isoprene yield.It provides an alternative for the recovery of organic matter resources from anaerobically digested sludge.
作者
张剑桥
杨敏
宁兹功
路璐
ZHANG Jianqiao;YANG Min;NING Zigong;LU Lu(Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,China;Luohu District Urban Management and Comprehensive Law Enforcement Bureau,Shenzhen 518003,China;State Key Laboratory of Urban Water Resource and Environment,School of Civil and Environmental Engineering,Harbin Institute of Technology,Shenzhen 518055,China)
出处
《工业水处理》
CAS
CSCD
北大核心
2023年第12期175-180,共6页
Industrial Water Treatment
基金
广东省自然科学基金项目(2022A1515012016)
深圳市科技计划资助项目(KQTD20190929172630447,JCYJ20210324124209025)。
关键词
厌氧消化污泥
有机物回收
生物合成
异戊二烯
合成生物学
anaerobically digested sludge
organics recovery
biosynthesis
isoprene
synthetic biology
