摘要
目的探究血清Krüppel样转录因子2(KLF2)、血管生成素1(Ang1)水平与新生儿呼吸窘迫综合征(NRDS)严重程度及预后的相关性。方法选取2019年1月至2022年12月在该院就诊的416例NRDS患儿为NRDS组,根据NRDS组患儿胸部影像检查结果将其分为轻度组(145例)、中度组(174例)和重度组(97例);再根据NRDS患儿结局将其分为预后良好组322例和预后不良组94例;另选取同期该院体检健康的早产儿150例作为对照组。采用酶联免疫吸附试验(ELISA)测定血清KLF2、Ang1水平;采用Pearson相关分析NRDS患儿血清KLF2、Ang1水平与新生儿急性心理学评分围生期补充Ⅱ(SNAPPE-Ⅱ)、1 min Apgar评分的相关性;采用受试者工作特征(ROC)曲线评价血清KLF2、Ang1水平在预测NRDS患儿预后中的价值。结果NRDS组出生体重及血清KLF2、Ang1水平较对照组降低(P<0.05);NRDS患儿血清KLF2、Ang1水平及1 min Apgar评分随患儿病情严重程度加重而逐渐降低(P<0.05);与预后良好组比较,预后不良组NRDS患儿血清KLF2、Ang1水平均降低,SNAPPE-Ⅱ评分升高(P<0.05);NRDS患儿血清KLF2、Ang1水平与SNAPPE-Ⅱ评分均呈负相关(P<0.05),与1 min Apgar评分均呈正相关(P<0.05);血清KLF2、Ang1水平预测NRDS患儿预后不良的曲线下面积(AUC)分别为0.931、0.909,二者联合预测NRDS患儿预后不良的AUC为0.949,高于KLF2、Ang1单独预测,且灵敏度为96.81%。结论血清KLF2、Ang1水平在NRDS患儿中均降低,二者水平均随NRDS患儿病情严重程度加重而逐渐降低,且二者在预测NRDS患儿预后中具有重要价值。
Objective To investigate the correlation of serum levels of Krüppel-like transcription factor 2(KLF2)and angiopoietin-1(Ang1)with the severity and prognosis of neonatal respiratory distress syndrome(NRDS).Methods A total of 416 children with NRDS who were treated in this hospital from January 2019 to December 2022 were selected as the NRDS group.According to the results of chest imaging examination,the NRDS group was divided into mild group(145 cases),moderate group(174 cases)and severe group(97 cases).According to NRDS outcome the children were divided into the good prognosis group 322 cases and bad prognosis group 94 cases,another 150 healthy premature infants were selected as the control group in the hospital.Serum KLF2,Ang1 levels were measured by enzyme-linked immunosorbent assay(ELISA).Pearson correlation was used to analyze the correlation between serum KLF2,Ang1 levels and neonatal acute physiology score perinatal supplementⅡ(SNAPPE-Ⅱ),1 min Apgar score in neonates with NRDS.The receiver operating characteristic(ROC)curve was used to evaluate the value of serum KLF2,Ang1 levels in predicting the prognosis of children with NRDS.Results Compared with the control group,the NRDS group had significantly lower birth weight and serum KLF2,Ang1 levels(P<0.05).In the children with NRDS,the serum of KLF2,Ang1 levels and 1 min Apgar score gradually decreased with the increase in the severity of the disease(P<0.05).Compared with the good prognosis group,the children with NRDS in the poor prognosis group had significant decreased in the serum KLF2 and Ang1 levels and a significant increased in the SNAPPE-Ⅱscore(P<0.05).In children with NRDS,the serum levels of KLF2 and Ang1 were negatively correlated with SNAPPE-Ⅱscore and positively correlated with 1 min Apgar score(P<0.05).The area under the curve(AUC)of serum KLF2 and Ang1 levels in predicting the poor prognosis of NRDS children were 0.931 and 0.909,respectively.The AUC of the combination of KLF2 and Ang1 in predicting the poor prognosis of NRDS children w
作者
颜海峰
霍开明
林裕庆
周家仍
YAN Haifeng;HUO Kaiming;LIN Yuqing;ZHOU Jiareng(Department of Pediatrics,the Second Affiliated Hospital of Hainan Medical College,Haikou,Hainan 570311,China)
出处
《国际检验医学杂志》
CAS
2023年第24期2996-3000,共5页
International Journal of Laboratory Medicine
基金
海南省卫生健康行业科研项目(琼卫科教[2020]9号389)。
