摘要
【目的】明确田间条件下施加氮(N)、磷(P)和钾(K)肥对玉米穗位叶内糖类、脂类和蛋白质代谢相关基因的表达模式与分子调控机制。【方法】以玉米“鲁豫16号”为材料,设置5个处理:不施肥(CK)、氮肥(N)、氮+磷肥(NP)、氮+钾肥(NK)和氮+磷+钾肥(NPK)。于灌浆期选取穗位叶的上半部分,利用RNA⁃Seq技术进行转录组分析,利用Hisat2等软件分析不同肥料处理下糖类、脂类和蛋白质代谢相关差异表达基因(Differentially expressed genes,DEGs)。【结果】GO(Gene ontology)功能富集分析发现,与糖类、脂类和蛋白质代谢相关的DEGs在生物过程(Biological process,BP)、细胞组成(Cell component,CC)和分子功能(Molecular function,MF)均有富集。尽管富集的条目相同,但在不同组合处理下DEGs数目各异。KEGG(Kyoto encyclopedia of genes and ge⁃nomes)通路富集分析表明,碳代谢、淀粉、蔗糖代谢和氨基酸合成途径中富集的DEGs数目较多。在不同施肥处理下,|log2 FC|>1且P<0.05的参与糖类、脂类和蛋白质氨基酸代谢的DEGs总数分别是82、29和84个。其中,|log2 FC|>2的DEGs总数分别是24、6和17个。DEGs数目以NPvsCK和NPKvsCK组合最多,NvsCK组合最少。对|log2 FC|>2的DEGs分析发现,这些DEGs在表达水平和表达模式存在显著差异。【结论】氮、钾、磷肥配施显著影响玉米叶片糖类、脂类和蛋白质代谢相关基因的转录水平,鉴定出了一些参与三大有机物质代谢的关键基因,为将来通过合理施肥提高玉米叶片光合利用率提供了分子方面的依据。
【Objective】The present paper aimed to clarify the expression pattern and molecular regulation mechanism of genes related to sug⁃ar,lipid and protein metabolism in maize ear leaves under different fertilization in field conditions.【Method】Maize cultivar‘Luyu 16’was used as the material and five treatments were set up:No fertilizer(CK),nitrogen fertilizer(N),N+P fertilizer(NP),N+K fertilizer(NK)and N+P+K fertilizer(NPK).At grain filling stage,the upper part of ear leaves was harvested for transcriptome analysis using RNA⁃Seq techniques.The differentially expressed genes(DEGs)under different fertilizer treatments were analyzed by Hisat2 software.【Result】GO(Gene ontology)analysis showed that DEGs related to sugar,lipid and protein metabolism were enriched in Biological process(BP),Cell components(CC)and Molecular function(MF).Although the enriched items were the same,the number of DEGs varied under different combinations.KEGG(Kyoto encyclopedia of genes and genomes)analysis exhibited that more DEGs were enriched in carbon metabolism,starch and sucrose metabolism,and amino acid synthesis pathways.The total number of DEGs with both|log2 FC|>1 and P<0.05 involved in sugar,lipid and protein metabolism under different fertilizer treatments was 82,29 and 84,respectively.Among them,the total number of DEGs with|log2 FC|>2 was 6,24 and 17.The number of DEGs showed the maximum in comparisons of NPvsCK and NPKvsCK but the minimum in NvsCK comparison.Further analysis of DEGs with|log2 FC|showed that these DEGs had significant differ⁃ences in the expression level and expression patterns.【Conclusion】The combined of nitrogen,potassium and phosphorus fertilizer signifi⁃cantly affected the transcription levels of genes related to sugar,lipid and protein amino acids metabolism in maize ear leaves.Some key genes participating in the three organic substances’metabolism were identified.The results provided molecular basis for improving the photo⁃synthetic efficiency of maize leaves by rational fertilizati
作者
曾黎
朱凯丽
刘立功
刘树堂
李军
ZENG Li;ZHU Kai-li;LIU Li-gong;LIU Shu-tang;LI Jun(College of Agronomy,Qingdao Agricultural University,Qingdao,Shandong 266109,China;Vegetable Research Institute,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;College of Resources and Environment,Qingdao Agricultural Uni-versity,Qingdao,Shandong 266109,China)
出处
《西南农业学报》
CSCD
北大核心
2023年第10期2126-2134,共9页
Southwest China Journal of Agricultural Sciences
基金
青岛农业大学教学案例库(QNYYAL1901)
国家重点研发计划课题(2017YFD0301002)。
关键词
玉米
转录组
氮肥
磷肥
钾肥
Maize
Transcriptome
Nitrogen fertilizer
Phosphorus fertilizer
Potassium fertilizer
