摘要
目的观察活体兔眼行筛板穿刺术后眼压的变化,明确筛板穿刺术作为新型降眼压方法的可行性。设计实验研究。研究对象健康成年新西兰兔4只(4眼)。方法筛选健康成年新西兰兔4只,随机选取3只为实验组行玻璃体切除和筛板穿刺并植入引流管,1只行单纯玻璃体切除术为对照,均以右眼接受手术,左眼不做任何处理。分别于术前、术后1~7天、18天、20天、21天、22天、24天、25天测定眼压,术后5天、9天、19天、26天进行一般眼科检查,术后2天、3天、7天行术眼B超检查。术后1个月所有动物双眼取材进行组织病理学检查,其中实验组取1只于处死前半小时行玻璃体腔注射经0.2μm滤膜过滤的日本墨水100μL。主要指标眼压及组织病理学结果。结果术后1周所有动物术眼眼压均降低,术后18天对照兔眼眼压逐渐升高至术前眼压水平,而实验组中的2只术眼在近一个月的观察期内平均眼压分别为(9.77±1.30)mmHg和(10.08±0.76)mmHg,且始终低于术前眼压16.00 mmHg、14.00 mmHg和对侧未处理眼眼压(14.85±1.46)mmHg和(15.65±1.93)mmHg。玻璃体腔注射墨水的实验兔眼组织学检查HE染色发现视神经血管旁间隙处有黑色墨迹沉积。结论兔眼实验研究初步证明了筛板穿刺可使眼压降低,筛板穿刺后房水可能通过神经血管旁路系统进入脑脊液。
Objective To determine the feasibility of lamina cribrosa puncture in intraocular pressure(IOP)reduction in rabbit eyes.Design Experiment study.Participants 4 healthy adult New Zealand rabbits(4 eyes).Methods Four healthy adult New Zealand rabbits were selected and 3 randomly for the experimental group to puncture lamina cribrosa and implant drainage tube.A sham rabbit eye with vitrectomy was used as a control.All surgeries were performed on the right eye only,with no treatment gived to the left eye.Intraocular pressure(IOP)was measured at pre-operative and postoperative 7 consecutive days,18th day,20th day,21st day,22nd day,24th day,25th day.Ophthalmic Ultrasound B was obtained at the 2nd day,3rd day,and 7th day after the operation.All 4 rabbits were sacrificed 1 month postoperatively,and the eyeballs were enucleated for histopathological examination and hematoxylin-eosin(HE)staining and one of the experimental eyes was injected 100μL of Japanese ink filtered by 0.2μm filter membrane to vitreous cavity half an hour before death.Main Outcome Measures IOP and histopathological result.Results IOP measurements of all animals decreased 1 week after the operation,and the control rabbit eye increased gradually to the baseline at the 18th day postoperatively.IOP measurements of 2 experimental eyes in the experimental group during the observation period of the past month were(9.77±1.30)mmHg and(10.08±0.76)mmHg respectively,and were always lower than the pre-operative intraocular pressure(16.00 mmHg and 14.00 mmHg)and the contralateral untreated eyes(14.85±1.46)mmHg and(15.65±1.93)mmHg.The HE staining showed an accumulation of Japanese ink in para-vascular spaces around blood vessel walls in the one experimental eye.Conclusion Experimental study in rabbit preliminarily shows it is possible to drainage aqueous humor through a hole of lamina cribrosa to subarachnoid space.
作者
陶妍
温良
郭超群
张绍丹
梁远波
吴文灿
王宁利
Tao Yan;Wen Liang;Guo Chaoqun;Zhang Shaodan;Liang Yuanbo;Wu Wencan;Wang Ningli(Institute of Glaucoma,Wenzhou Medical University,State Clinical Research Center for Eye Diseases,The Eye Hospital of Wenzhou Medical University,Wenzhou 325000,China;Beijing Institute of Ophthalmology,Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University,Beijing Key Laboratory of Ophthalmology and Visual Science,Beijing 100730,China)
出处
《眼科》
CAS
2023年第6期462-468,共7页
Ophthalmology in China
基金
国家自然科学基金面上项目(81770919)
浙江省自然科学基金(LY17H120004)。
关键词
筛板
青光眼
新西兰兔
lamina cribrosa
glaucoma
New Zealand rabbit
