摘要
为研究质子辐射对人恶性黑色素瘤的杀伤效应,本研究利用北京HI-13串列加速器提供的15 MeV质子,以0、1、2、4、8 Gy剂量辐照A375细胞,以细胞克隆术和流式细胞术检测A375细胞的克隆形成率、周期阻滞及凋亡率,用免疫荧光法检测2 Gy辐照后细胞的γH2AX焦点数,并与相同条件下的γ射线辐射对比。结果表明,在1~8 Gy剂量下,随着剂量的增加,A375细胞的存活率下降,在4~8 Gy剂量下,细胞的存活率明显低于γ射线。辐照后12 h,细胞G2/M期阻滞随剂量的增加而增加,质子辐射诱导的周期阻滞强于γ射线;辐照后48 h,γ射线诱导的细胞周期阻滞已基本解除,但质子诱导的细胞周期阻滞除1 Gy外,2~8 Gy均未完全解除。辐射诱导的细胞凋亡随照射剂量的增加而增加,随着时间的延长,凋亡比例有所增加,且质子诱导的细胞凋亡率高于γ射线。辐照2 Gy后,γ射线和质子诱导的γH2AX焦点峰值均在照后1 h出现,质子辐射诱导的γH2AX焦点数和大小均高于γ射线。以上结果表明,质子辐射可有效杀伤恶性黑色素瘤A375细胞,在黑色素瘤治疗中有潜在应用价值。
Malignant melanoma is a highly invasive skin cancer that can rapidly metastasize and is often resistant to traditional chemotherapy and radiotherapy.In recent years,the incidence and mortality rates of malignant melanoma are increasing,especially among young people.Therefore,there is an increasing need to develop effective therapies for this deadly disease.Proton therapy is a promising cancer treatment that uses protons to target and destroy cancer cells.Compared to traditional radiation therapy,the advantage of proton therapy is that it can provide highly concentrated radiation doses to tumors while minimizing damage to surrounding healthy tissue.However,to fully exploit the potential of proton therapy,it is important to understand the biological effects of proton radiation on cancer cells and normal tissues.In this study,A375 cells were irradiated with 15 MeV protons andγ-ray at different doses(0,1,2,4,and 8 Gy)to investigate the effects of different radiation types and doses on A375 cell survival,cell cycle progression,cell apoptosis,and DNA damage.The results show that as the dose increases from 1 Gy to 8 Gy,the survival rate of A375 cells decreases,and cell survival induced by 4-8 Gy proton radiation is significantly lower than that induced byγ-ray.These findings indicate that proton radiation is more effective in killing cancer cells than traditional radiation therapy.Additionally,the study shows that radiation-induced G2/M phase arrest increases with dose,with proton-induced cell cycle arrest stronger than that induced byγ-ray.Forty-eight hours after irradiation,the cell cycle arrest induced byγ-ray is mostly released,but the cell cycle arrest induced by proton radiation is not completely released at 2-8 Gy except for 1 Gy.This suggests that proton radiation has a more sustained effect on cancer cells,making it a potentially more effective treatment option for malignant melanoma.The study also shows that cell apoptosis induced by ionizing radiation increases with increasing irradiation dose,and the pro
作者
王巧娟
隋丽
刘建成
汪越
马立秋
朱润
郭刚
WANG Qiaojuan;SUI Li;LIU Jiancheng;WANG Yue;MA Liqiu;ZHU Run;GUO Gang(China Institute of Atomic Energy,Beijing 102413,China;National Innovation Center of Radiation Application,Beijing 102413,China)
出处
《原子能科学技术》
EI
CAS
CSCD
北大核心
2023年第12期2455-2466,共12页
Atomic Energy Science and Technology
基金
稳定基础科研计划(BJ19001112)
中国原子能科学研究院青年英才培育基金(11YC202212000201)。
