摘要
该文制备了一种新型冠状病毒拉曼标记免疫层析试纸,该试纸采用Au-MGITC@SiO_(2)核壳结构作为探针,并使用该试纸对SARS-CoV-2、229E、NL63、OC43、PR8等多种呼吸道病毒进行检测,通过观察T线显色情况和T线拉曼光谱测试结果,对该试纸的性能进行评价。结果表明:制备的试纸具有较高的灵敏度与特异性,其SARS-CoV-2拉曼探测极限可达3.5×10^(–1) PFU/mL,且对于229E、NL63、OC43三种冠状病毒和PR8流感病毒这4种呼吸道病毒均未发生交叉反应。对于T线显色不明显的弱阳性情况,可以通过拉曼光谱检测作出进一步判别。该文的实验结果可为拉曼标记免疫层析技术在SARS-CoV-2快速检测的应用提供参考。
In this paper,the novel coronavirus Raman-labeled immunoassay test strips were prepared with a probe of Au-MGITC@SiO_(2)core-shell structure.Various respiratory viruses including SARS-CoV-2,229E,NL63,OC43,PR8 were detected by using the test strips.The performance of the test strips was evaluated by testing the coloration and Raman spectrum of the T-line.The experimental results demonstrate the high sensitivity and specificity of our test strips.The SARS-CoV-2 Raman detection limit of 3.5×10^(–1) PFU/mL was detected.The test strips don’t react with the three coronaviruses 229E,NL63,OC43 and PR8 influenza virus.For the weak positive case,the T-line coloration is not obvious,the Raman spectrum detection is required for further discriminating.This work provides an new detection technology of SARS-CoV-2,which may be useful for the application of Raman labeled immunochromatography in the rapid detection of SARS-CoV-2.
作者
祁道健
莫文博
李兆国
乐玮
黄景林
李佳
倪爽
周民杰
QI Daojian;MO Wenbo;LI Zhaoguo;LE Wei;HUANG Jinglin;LI Jia;NI Shuang;ZHOU Minjie(Laser Fusion Research Center,CAEP,Mianyang 621900,China;Department of Engineering Physics,Tsinghua University,Beijing 100084,China)
出处
《中国测试》
CAS
北大核心
2023年第11期45-52,共8页
China Measurement & Test
关键词
新型冠状病毒
表面增强拉曼光谱技术
标记
免疫层析
severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)
surface-enhanced Raman spectroscopy technology
label
immunochromatography
