摘要
叶绿素的检测和监测在植物生理、水质状况、农业管理和生态系统健康等方面具有重要意义。笔者设计了一种基于微波光子滤波器(MPF)射频强度的叶绿素检测系统,该系统利用叶绿素质量浓度变化导致光纤端面反射率发生变化的原理,根据射频强度与叶绿素质量浓度之间的关系,实现对叶绿素的检测。该系统具有体积小、抗干扰能力强的特点,而且可以实现长距离检测。在该系统中,宽谱光源、微波信号源、掺铒光纤放大器、电光调制器、隔离器、光耦合器、光电探测器、频谱仪、光纤等构成了迈克耳孙干涉仪结构的微波光子滤波器。提取不同蔬菜的叶绿素溶液,采用该系统对叶绿素溶液进行检测,验证了其在植物叶绿素检测方面的可行性。采用该系统检测了叶绿素实验原液以及由叶绿素标准溶液配制的不同质量浓度梯度的叶绿素溶液,验证了射频强度与叶绿素质量浓度之间存在较好的线性关系,其中最大射频强度与叶绿素质量浓度的线性拟合度(R2)达到了0.9741,灵敏度为0.007881 d B/(μg·L^(-1)),最小射频强度与叶绿素质量浓度的线性拟合度达到了0.9841,灵敏度为0.02258 d B/(μg·L^(-1))。上述实验结果表明该系统在高质量浓度叶绿素和低质量浓度叶绿素情况下均具有较好的传感性能。
Objective Chlorophyll detection and monitoring play crucial roles in plant physiology,water quality,agricultural management,and ecosystem health.Based on their principles and application characteristics,current chlorophyll detection methods can be classified into several categories such as spectroscopic measurements(such as spectrophotometry and fluorescence techniques),compositional analysis(such as high-performance liquid chromatography,HPLC),and morphological observation(such as microscopic counting and high-resolution spectral imaging).However,many of these methods present various drawbacks.Spectrophotometric methods involve cumbersome detection steps and suffer from low sensitivity;the HPLC method takes a long time and is costly;the fluorescence method does not work well with high-concentration solutions;some detection equipment demands on-site measurement,hindering long-distance detection;and real-time capabilities are often lacking,demanding significant manpower and resources.In recent times,microwave photonics(MWP)technology evolves rapidly.Microwave photonic filters(MPF),pivotal technologies within this realm,capitalize on the fiber optic sensor’s resilience to high pressure,high temperature,corrosion,and electromagnetic interference.These sensors process RF signals and manage optical carrier signal processing within the optical domain.This study introduces a chlorophyll detection system rooted in the microwave photonic filter RF intensity.This novel detection method stands out for its compactness,robust anti-interference capabilities,and potential for long-distance detection.The focus here is the application of microwave photonic filters for chlorophyll detection.Our experiment underscores the feasibility of this principle and highlights a pioneering approach to chlorophyll detection with expansive application prospects.Methods The system comprised a broad-spectrum light source(ASE),a microwave signal source(RF),an optical fiber amplifier(EDFA),an electro-optical modulator(EOM),an isolator(ISO),an opt
作者
李乾坤
丁美琪
桂林
连枭轩
钱敏
Li Qiankun;Ding Meiqi;Gui Lin;Lian Xiaoxuan;Qian Min(School of Intelligent Manufacturing and Control Engineering,Shanghai Polytechnic University,Shanghai 201209,China;School of Computer and Information Engineering,Shanghai Polytechnic University,Shanghai 201209,China)
出处
《中国激光》
EI
CAS
CSCD
北大核心
2023年第21期221-231,共11页
Chinese Journal of Lasers
基金
上海第二工业大学电子信息学位点2021年专项建设项目(A30DB218802-0402)。
关键词
传感
叶绿素
叶绿素质量浓度检测
微波光子滤波器
微波光子传感
光纤传感
sensing
chlorophyll
chlorophyll concentration detection
microwave photonic filters
microwave photonic sensing
fiber optic sensing
