摘要
Fluorescence detection is widely used in biology and medicine,while the realization of on-chip fluorescence detection is vital for the portable and point-of-care test(POCT)application.In this Letter,we propose an efficient fluorescence excitation and collection system using an integrated GaN chip consisting of a slot waveguide and a one-dimensional photonic crystal(1D PC)waveguide.The slot waveguide is used to confine the excitation light for intense light–sample interaction,and the one-trip collection efficiency at the end of slot waveguide is up to 14.65%.More interestingly,due to the introduction of the 1D PC waveguide,the fluorescence signal is directly filtered out,and the excitation light is reflected to the slot waveguide for multiple excitations.Its transmittances for the designed exciting wavelength of 520 nm and the fluorescent wavelength of 612 nm are 0.2%and 85.4%,respectively.Finally,based on numerical analysis,the total fluorescence collection efficiency in our system amounts to 15.93%.It is the first time,to our knowledge,that the concept of an all-in-one-chip fluorescence detection system has been proposed,which paves the way for on-chip fluorescence excitation and collection,and may find potential applications of miniaturized and portable devices for biomedical fluorescence detection.
作者
张家晖
徐峰
安然
王琳
江敏
王光辉
陆延青
Jiahui Zhang;Feng Xu;Ran An;Lin Wang;Min Jiang;Guanghui Wang;Yanqing Lu(College of Engineering and Applied Sciences,Nanjing University,Nanjing 210093,China;Key Laboratory of Intelligent Optical Sensing and Integration of the Ministry of Education,Nanjing University,Nanjing 210009,China;College of Science,Wuxi University,Wuxi 214411,China)
基金
supported by the National Natural Science Foundation of China(Nos.61875083 and 61535005)
the Social Development Project of Jiangsu Province(No.BE2019761)
the Key Research and Development Program of Shandong Province(No.2020CXGC011304).
