摘要
目的:分析儿童支气管哮喘(BA)患者中血清趋化因子配体19(CCL19)和趋化因子配体21(CCL21)的表达,探讨其在儿童BA中可能介导的病理过程及其在哮喘中是否具有临床指导意义。方法:收集2021年7月至2022年12月于包头市第四医院就诊的BA儿童共72例作为哮喘组,其中急性发作期(AA)32例,临床缓解期(CR)40例;同期选取正常体检儿童33例作为正常对照组(HC)。所有入组儿童均采用ELISA法检测CCL19、CCL21水平,行统计学比较,并评价CCL19、CCL21在儿童支气管哮喘中的诊断效能。结果:(1)各组间血清CCL19、CCL21表达水平比较,差异有统计学意义(P<0.05),其中哮喘组血清CCL19、CCL21表达水平均高于HC组,且AA组又高于CR组;(2)BA儿童CCL19、CCL21水平呈正相关(r=0.819,P<0.05);(3)ROC曲线结果显示,CCL19诊断曲线下面积(AUC)为0.797,最佳截断值为131.58 pg/mL,其敏感度为79.2%,特异度为69.7%;CCL21的AUC为0.771,最佳截断值为307.33 pg/mL,其敏感度及特异度分别为69.4%、84.8%。结论:(1)血清CCL19和CCL21表达水平在支气管哮喘患儿中明显升高,且两者间呈正向相关,提示CCL19与CCL21可能参与哮喘发病机制的过程;(2)ROC曲线提示血清CCL19与CCL21具有良好的诊断效能,提示二者可能成为诊断哮喘及评估临床治疗效果的一个新的实验室依据。
Objective:To analyze the expression of serum C-C chemokine ligand 19(CCL19)and C-C chemokine ligand 21(CCL21)in children with bronchial asthma(BA),and to explore its possible mediated pathological process in children with BA,and whether it has clinical guiding significance in asthma.Methods:A total of 72 BA children who were treated in Baotou Fourth Hospital from July 2021 to December 2022 were collected as the asthma group,including 32 cases of acute exacerbation(AA)and 40 cases of clinical remission(CR).At the same time,33 children with normal physical examination were selected as normal control group(HC).The levels of CCL19 and CCL21 were detected by ELISA in all enrolled children,and the diagnostic efficacy of CCL19 and CCL21 in children with bronchial asthma was evaluated.Results:(1)There were significant differences in the expression levels of serum CCL19 and CCL21 among the three groups(P<0.05),while the expression levels of serum CCL19 and CCL21 in the asthma group were higher than those in the HC group,and those in the AA group were higher than those in the CR group.(2)The levels of CCL19 and CCL21 in BA children were positively correlated(r=0.819,P<0.05).(3)The results of ROC curve showed that the area under the curve(AUC)of CCL19 was 0.797,the optimal cut-off value was 131.58 pg/mL,the sensitivity was 79.2%,and the specificity was 69.7%.The AUC of CCL21 was 0.771,the optimal cut-off value was 307.33 pg/mL,and the sensitivity and specificity were 69.4%and 84.8%,respectively.Conclusion:(1)The expression levels of serum CCL19 and CCL21 are significantly increased in children with bronchial asthma,and there is a positive correlation between them,suggesting that CCL19 and CCL21 may be involved in the pathogenesis of asthma.(2)The ROC curve shows that serum CCL19 and CCL21 had good diagnostic efficacy,suggesting that they may become a new laboratory basis for the diagnosis of asthma and the evaluation of clinical treatment.
作者
王崴
贾春梅
曲艳杰
宁立华
陈蕊
WANG Wei;JIA Chunmei;QU Yanjie;NING Lihua;CHEN Rui(Baotou Medical College,Inner Mongolia University of Science&Technology,Baotou 014040,China;Baotou Fourth Hospital)
出处
《包头医学院学报》
CAS
2023年第12期66-70,共5页
Journal of Baotou Medical College
