摘要
目的通过胸腺法新与NSCLC患者外周血来源的髓源性抑制细胞共同培养,观察胸腺法新对髓源性抑制细胞亚群的影响,探索其可能的作用机制。方法收集2021年1月至2022年12月江西省胸科医院经病理组织学或细胞学检查确诊的30例NSCLC患者的外周静脉血和肿瘤组织以及30例健康志愿者的外周静脉血。以HLA-DR^(-)CD14^(-)CD33^(+)作为NSCLC患者的髓源性抑制细胞标志,运用流式细胞仪检测并分析NSCLC患者外周血单个核细胞(PBMNC)、肿瘤组织髓源性抑制细胞的表达及外周血单个核细胞与胸腺法新共培养前后髓源性抑制细胞比例的变化。结果NSCLC患者手术前外周血中髓源性抑制细胞表达为(1.62±0.46)%,明显高于健康志愿者的(0.41±0.14)%,差异有统计学意义(P<0.01);与健康组外周血中髓源性抑制细胞表达比较,NSCLC患者肿瘤组织中表达明显增高,两者分别是(0.41±0.14)%、(0.96±0.39)%,差异有统计学意义(P<0.01);NSCLC患者肿瘤组织中髓源性抑制细胞表达低于外周血中的表达,两者分别是(0.96±0.39)%、(1.62±0.46)%,差异有统计学意义(P<0.01)。胸腺法新与NSCLC患者外周血单个核细胞共培养前后,髓源性抑制细胞表达分别是(1.62±0.46)%、(0.53±0.11)%,差异有统计学意义(P<0.01)。结论髓源性抑制细胞参与NSCLC疾病进展过程,胸腺法新与NSCLC患者外周血共培养后髓源性抑制细胞表达下降,表示胸腺法新可能通过促进其分化成熟而调节患者的免疫功能。
Objective To study the effect of thymic method on the subset of marrow-derived inhibitory cells and explore the possible mechanism of action,by co-culture of thymic method with peripheral blood-derived inhibitory cells from NSCLC patients.Methods Peripheral venous blood and tumor tissues from 30 patients with NSCLC and 30 healthy volunteers were collected.With HLA-DR^(-)CD14^(-)CD33^(+) as the marker of NSCLC patients,the expression of peripheral blood mononuclear cells(PBMNC),tumor tissue and analyzed by flow cytometry in NSCLC patients.Results The expression of marrow-derived inhibitory cells in peripheral blood of NSCLC patients was significantly higher(1.62±0.46)%than that of healthy volunteers(0.41±0.14)%,there had statistical difference(P<0.01);Compared with the expression of myeloid-derived inhibitory cells in the peripheral blood of the healthy group,Significant higher expression in the tumor tissues of NSCLC patients,Both are(0.41±0.14)%and(0.96±0.39)%,there had statistical difference(P<0.01);Lower expression of myelogenous inhibitory cells in tumor tissues of NSCLC patients than in peripheral blood,Both are(0.96±0.39)%and(1.62±0.46)%,there had statistical difference(P<0.01),respectively.Before and after co-culture of thymic method and peripheral blood mononuclear cells of NSCLC patients,the expression of myelogenous inhibitory cells was(1.62±0.46)%and(0.53±0.11)%(P<0.01).Conclusion Myeloid suppressor cells participate in the disease progression of NSCLC,and the expression of thymic suppressor cells decreased after coculture with peripheral blood of NSCLC patients,indicating that thymic neoplasia may regulate the immune function of patients by promoting their differentiation and maturation.
作者
史芳
邱慧萍
严进锦
柯昌林
李瑶
SHI Fang;QUI Huiping;YAN Jinjing(Jiangxi Provincal Chest Hospital,Nanchang,330006)
出处
《实用癌症杂志》
2023年第12期1972-1973,1977,共3页
The Practical Journal of Cancer
基金
江西省卫生健康委科技计划项目(编号:202130974)。
