摘要
目的探讨LINC00958在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中的表达和作用。方法利用TCGA数据库分析LINC00958在HNSCC中的表达和对生存预后的影响;体外培养HNSCC细胞系CAL27和HN6,对其转染对照组和敲低组(si-LINC00958)质粒,采用实时荧光定量PCR实验检测LINC00958表达;CCK-8实验检测细胞增殖能力变化;Transwell实验检测细胞迁移和侵袭能力变化;WB实验检测转移相关蛋白E-钙黏附蛋白(E-cadherin)、N-钙黏附蛋白(N-cadherin)和波形蛋白(Vimentin)表达情况;应用TargetScan数据库预测LINC00958和miR-877-3p的结合位点,双荧光素酶报告基因实验验证;分析miR-877-3p对HNSCC细胞增殖、迁移和侵袭能力的影响。结果LINC00958在HNSCC中高表达,并影响患者总体生存率(P<0.05)。和对照组(si-NC)相比,si-LINC00958组细胞增殖、迁移和侵袭能力降低,E-cadherin表达降低,N-cadherin和Vimentin表达升高(P<0.05);TargetScan数据库显示miR-877-3p是LINC00958的靶基因,双荧光素酶报告基因实验验证两者存在靶向关系。和对照组(NC)相比,miR-877-3p mimics组细胞增殖、迁移和侵袭能力显著降低(P<0.05)。结论LINC00958通过靶向调控miR-877-3p促进HNSCC的增殖、迁移和侵袭。
Objective To explore the expression and role of LncRNA LINC00958 in head and neck squamous cell carcinoma(HNSCC).Methods The expression of LINC00958 in HNSCC and its effect on survival and prognosis were analyzed using TCGA database.HNSCC cell lines CAL27 and HN6 were cultured in vitro,and the transfected control group and knockdown group(siLINC00958)plasmid were detected by real-time quantitative PCR and western blotting(WB)assay.The cell proliferation ability was detected by CCK-8 assay.The changes of cell migration and invasion ability were detected by transwell assay.The expressions of transferrelated proteins E-cadherin,N-cadherin and vimentin were detected by WB assay.The binding sites of LINC00958 and miR-877-3p were predicted by TargetScan database,and verified by double luciferase reporter gene assay.The effects of miR-877-3p on proliferation,migration and invasion of HNSCC cells were analyzed.Results LINC00958 was highly expressed in HNSCC and affected the overall survival rate(P<0.05).Compared with the control group(siNC),the proliferation,migration and invasion of cells in siLINC00958 group were decreased;the expression of E-cadherin was decreased,and the expression of N-cadherin and Vimentin was increased(P<0.05).The TargetScan database showed that miR-877-3p was the target gene of LINC00958,and the targeting relationship between miR-877-3p and LINC00958 was verified by double luciferase reporter gene assay.Compared with the control group(NC),cell proliferation,migration and invasion in miR-877-3p mimics group were significantly decreased(P<0.05).Conclusion LINC00958 promotes the proliferation,migration and invasion of HNSCC by targeting miR-877-3p.
作者
吉幻
李萌
姚恩惠
钟旖
张雨垚
武和明
李斌
JI Huan;LI Meng;YAO Enhui;ZHONG Yi;ZHANG Yuyao;WU Heming;LI Bin(Department of Oral and Maxillofacial Surgery,The Affiliated Stomatological Hospital of Nanjing Medical University,Jiangsu Province Key Laboratory of Oral Diseases,Jiangsu Province Engineering Research Center of Stomatological Translational Medicine,Nanjing 210029,China)
出处
《口腔医学》
CAS
2023年第11期968-974,共7页
Stomatology
基金
国家自然科学基金(82203580)
江苏省卫健委医学科研项目(M2020003)
江苏省科教能力提升工程——江苏省研究型医院(YJXYYJSDW4)
江苏省医学创新中心(CXZX202227)
南京市卫健委课题(YKK20247)。
