摘要
系统的研究了不同周期的纳米凹坑和纳米柱孔氧化铝膜表面直接修饰当归多糖(ASP)或通过γ-氨丙基三乙氧基硅烷(KH550)共价连接修饰ASP后,乳腺癌细胞MDA-MB-231在其表面生长的细胞形态和活性规律。结果发现,当归多糖直接修饰纳米凹坑和纳米柱孔结构能够有效抑制乳腺癌细胞的活性,对细胞形态影响较小。但纳米凹坑结构的细胞活性抑制效果优于纳米柱孔结构,纳米凹坑在300 nm周期时具有最优的细胞活性抑制效果,抑制率为27.7%。用KH550共价修饰ASP的纳米凹坑和纳米柱孔结构对细胞活性的抑制效果要优于直接修饰ASP时的抑制效果,细胞形态也发生了明显的变化,胞体周围生成大量板状伪足。纳米凹坑结构上的细胞活性抑制效果要优于纳米柱孔结构,纳米凹坑在300 nm周期时抑制效果最佳,抑制率为28.2%。该结果对于药物的设计以及输运具有一定的指导作用。
The surface of nanodent and nanopore structured alumina membranes with different periods were directly modified by Angelica sinensis polysaccharide(ASP)or covalently modified by ASP viaγ-aminopropyl triethoxysilane(KH550).After that,the morphology and cell viability of breast cancer cells MDA-MB-231 grown on the modified nanostructured surfaces were studied.It is found that nanodent and nanopore structure directly modified by ASP can effectively inhibit the viability of breast cancer cells,and have little effect on cell morphology.However,the inhibitory effect of nanodent structure on cell viability is superior to that of nanopore structure.Nanodent structure with period of 300 nm have the best inhibitory effect on cell viability,and the inhibitory rate is 27.7%.The nanodent and nanopore structures covalently modified by ASP via KH550 have a better inhibitory effect on cell viability than that directly modified ASP,and the cell morphology change obviously.A large number of lamellipodia are generated around the cell body.However,the inhibition effect of the nanodent structure on cell viability is better than that of the nanopore structure.Nanodent structure with period of 300 nm have the best inhibitory effect,and the inhibition rate is 28.2%.
作者
张智颖
梁瑞清
陈俊霖
梅晁源
李娟
孙润广
Zhang Zhiying;Liang Ruiqing;Chen Junlin;Mei Chaoyuan;Li Juan;Sun Runguang(School of the Physics and Information Technology,Shaanxi Normal University,Xi’an 710119,China)
出处
《稀有金属材料与工程》
SCIE
EI
CAS
CSCD
北大核心
2023年第11期3947-3953,共7页
Rare Metal Materials and Engineering
基金
国家自然科学基金(21965030)
陕西省重点研发计划(2023-YBGY-477)。
关键词
阳极氧化铝
当归多糖
细胞形态
细胞活性
抗肿瘤
Angelica sinensis polysaccharide
anodic aluminum oxide
cell morphology
cell viability
anti-tumor
