摘要
目的研究细胞核内巨噬细胞集落刺激因子(M-CSF)对宫颈癌细胞增殖能力和侵袭能力的影响及其可能机制。方法以构建的核内稳定表达M-CSF的HeLa细胞系为模型,溴脱氧尿苷(BrdU)标记的脱氧核糖核酸(DNA)复制分析法检测细胞增殖能力,Transwell小室检测细胞侵袭能力;蛋白免疫印迹(Western blot)检测细胞核蛋白核内转录因子κB p65(NF-κB p65)和细胞总蛋白基质金属蛋白酶2(MMP-2)的表达情况。结果核内稳定表达M-CSF的HeLa细胞的增殖能力和侵袭能力均明显增强(P<0.05,P<0.01),且NF-κB p65、MMP-2表达增多(均P<0.05)。结论核内M-CSF可促进宫颈癌细胞的增殖和侵袭能力,其机制与上调NF-κB p65和MMP-2表达有一定的关系。
Objective To study the effect of macrophage colony stimulating factor(M-CSF)in the nucleus on the proliferation and invasion ability of cervical cancer cells and its possible mechanisms.Methods Using the HeLa cell line with stable expression of M-CSF in the nucleus as the model,the proliferation ability of the cells was detected using bromodeoxyuridine(BrdU)labeled deoxyribonucleic acid(DNA)replication analysis,and the invasiveness of the cells was detected using Transwell cells;Western blot was used to detect the expression of nuclear protein transcription factorκB p65(NF-κB p65)and total cell protein matrix metalloproteinase-2(MMP-2).Resultsl The proliferation and invasion ability of HeLa cells stably expressing MCSF in the nucleus were significantly enhanced(P<0.05,P<0.01),and the expression of NF-κB p65 and MMP-2 was increased(all P<0.05).Conclusions Intranuclear MCSF can promote the proliferation and invasion ability of cervical cancer cells,and its mechanism is related to the upregulation of NF-κB p65 and MMP-2 expression.
作者
滕淑静
宋旭日
覃秋莲
Teng Shujing;Song Xuri;Qin Qiulian(Medical School of Yueyang Vocational Technical College,Yueyang 414000,China)
出处
《中国医师杂志》
CAS
2023年第11期1673-1677,共5页
Journal of Chinese Physician
基金
湖南省自然科学基金项目(2020JJ7071)。
关键词
巨噬细胞集落刺激因子
宫颈肿瘤
细胞增殖
肿瘤侵袭
Macrophage colony-stimulating factor
Uterine cervical neoplasms
Cell proliferation
Neoplasm invasiveness
