摘要
研究旨在通过动物试验,探究各剂量姜黄白芍复合物灌胃对老龄小鼠的抗氧化能力的影响。将SPF级老龄小鼠随机分为4组,为空白对照、低、中、高剂量[0.13,0.27和0.80 g/(kg·BW)]组,连续灌胃34 d后,分别测定各组体重、过氧化脂质含量、蛋白质羰基含量、抗氧化酶活力、还原型谷胱甘肽含量。与空白对照组比较,该受试物在中、高剂量组均能显著降低血清中8-异前列腺素含量(P<0.05、P<0.001),提高全血谷胱甘肽过氧化物酶活力(P<0.05、P<0.01),提高肝组织还原型谷胱甘肽含量(P<0.05、P<0.01),其中高剂量组能进一步降低血清丙二醛含量(P<0.05),提高肝组织还原型谷胱甘肽含量(P<0.01)。干预期间该受试物对老龄小鼠体重增长无不良影响。基于原国家食品药品监督管理局颁布的抗氧化功能评价方法(国食药监保化[2012]107号)的判定标准,认定姜黄白芍复合物具有抗氧化功能。
The aim of this study was to investigate the effect of gavage of various doses of curcumin and peony complexes on the antioxidant capacity of aging mice through animal experiments.The aging mice were randomly divided into four groups as blank control,low,medium,and high dose[0.13,0.27 and 0.80 g/(kg·BW)]groups,and after 34 d of consecutive gavage,the body weight,lipid peroxide content,protein carbonyl content,antioxidant enzyme activity,and reduced glutathione content were measured in each group,respectively.Compared with the blank control group,this subject in both medium and high dose groups could signifcantly reduce the serum 8-isoprostane content(P<0.05;P<0.001),increase the whole blood glutathione peroxidase activity(P<0.05;P<0.01),and increase the hepatic tissue reduced glutathione content(P<0.05;P<0.01),of which the high dose group could further reduce the serum malondialdehyde content(P<0.05)and increase the hepatic tissue reduced glutathione content(P<0.01).The subject had no adverse effect on body weight gain in aging mice during the intervention period.Based on the adjunctive antioxidant function evaluation method promulgated by the former State Food and Drug Administration([2012]No.107),it was concluded that curcumin and peony complexes had antioxidant function.
作者
殷光玲
肖义然
贤欢
殷环
YIN Guanglin;XIAO Yiran;XIAN Huan;YIN Huan(Institute of Nutrition&Health,BYHEALTH Co.,Ltd.,Guangzhou 510663)
出处
《食品工业》
CAS
2023年第11期93-97,共5页
The Food Industry
