摘要
目的 探讨柴芩承气汤(chaiqin chengqi decoction, CQCQD)对急性胰腺炎(acute pancreatitis, AP)小鼠血清脂质代谢的影响。方法 将27只C57BL/6小鼠按照每组9只随机分成3组。AP模型组(AP组)与AP模型加CQCQD治疗组(APQ组)均采用间隔1 h共7次的雨蛙素(50μg/kg)腹腔注射以诱导AP模型,对照组(CON组)则接受同频次、等体积的磷酸盐缓冲液腹腔注射。APQ组小鼠在第1次注射雨蛙素后1 h开始接受间隔4 h共3次的CQCQD(5.5 g/kg)灌胃干预,AP组仅给予等量蒸馏水灌胃。第1次雨蛙素或磷酸盐缓冲液注射后12 h收集血液和组织样本,检测各组小鼠血清淀粉酶、脂肪酶、胰腺病理改变情况以及胰腺组织髓过氧化物酶活性以综合评价AP模型严重程度及CQCQD疗效,并分析各组小鼠的血清脂质代谢产物。结果 与CON组相比,AP组小鼠胰腺组织水肿、炎症细胞浸润和坏死明显,血清淀粉酶、脂肪酶、胰腺髓过氧化物酶水平升高(P<0.05);与AP组相比,APQ组胰腺炎症浸润及坏死均有显著改善,血清淀粉酶下降(P<0.05)。血清脂质代谢组学共鉴定319种代谢物,共有13种脂质代谢物在AP组显著上调并在APQ组显著下降,其中9种属于甘油磷脂代谢途径中的溶血磷脂酰乙醇胺(lyso-phosphatidylethanolamine, LPE)分子,进一步统计分析发现其中6种LPE分子可作为潜在的生物标志物。结论 CQCQD能够有效改善雨蛙素诱导AP小鼠的胰腺损伤和血脂代谢紊乱,显著改善AP小鼠血清LPE水平的异常升高。然而,LPE在AP中的作用以及CQCQD对LPE代谢的调控机制仍需进一步的研究。
Objective To investigate the effect of chaiqin chengqi decoction(CQCQD)on serum lipid metabolism in experimental acute pancreatitis.Methods A total of 27 C57BL/6 mice were randomly divided into three groups(n=9 for each group).The mice in the acute pancreatitis model group(AP group)and the acute pancreatitis model+CQCQD treatment group(APQ group)received seven intraperitoneal injections of cerulein(50µg/kg)at hourly intervals,while the mice in the control group(CON group)received phosphate-buffered saline injections at the same regimen of cerulein.Oral gavage of CQCQD(5.5 g/kg)or same volume of distilled water was commenced 1 h after the first cerulein injection for three times at intervals of 4 h for the APQ group and AP group,respectively.Animals were sacrificed 12 h after the first cerulein/phosphate-buffered saline injection for collecting serum and tissue samples.The levels of serum lipase and amylase,pancreatic histopathology assessment,as well as pancreatic myeloperoxidase activity,were used to assess the severity of acute pancreatitis and the efficacy of CQCQD.Additionally,serum lipid metabolites were analyzed in all groups.Results In comparison to the CON group,the mice in the AP group exhibited significant edema,inflammatory cell infiltration,necrosis of pancreatic tissues,as well as elevated levels of serum amylase,lipase,and pancreatic myeloperoxidase activity(P<0.05);in comparison to the AP group,inflammatory cell infiltration and necrosis of pancreatic tissue,as well as elevated level of serum amylase significantly reduced in the APQ group(P<0.05).A total of 319 lipid molecules were identified in serum,and 13 lipid metabolites were significantly increased in the AP group and successfully decreased in the APQ group,of which 9 were lyso-phosphatidylethanolamine(LPE)molecules involved in the glycerol phospholipid metabolic pathway.Further statistical analysis revealed that six of these LPE molecules could serve as potential biomarkers.Conclusions CQCQD ameliorated pancreatic injury and serum lipid met
作者
李玉莹
文泳鉴
钟少琦
杨鑫敏
姚林波
王雯
刘婷婷
黄伟
孙鑫
夏庆
LI Yuying;WEN Yongjian;ZHONG Shaoqi;YANG Xinmin;YAO Linbo;WANG Wen;LIU Tingting;HUANG Wei;SUN Xin;XIA Qing(West China Center of Excellence for Pancreatitis,Institute of Integrated Traditional Chinese and Western Medicine,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China;West China Biobanks,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China;Chinese Evidence-based Medicine Center,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China)
出处
《华西医学》
CAS
2023年第11期1701-1708,共8页
West China Medical Journal
基金
国家自然科学基金(82274321,82225049,81973632,82270672)
四川省自然科学基金(2023NSFSC0695)。
关键词
急性胰腺炎
柴芩承气汤
脂质组学
溶血磷脂酰乙醇胺
动物模型
Acute pancreatitis
chaiqin chengqi decoction
lipidomics
lyso-phosphatidylethanolamine
animal models
