摘要
目的探讨口腔腺样囊性癌(ACC)患者血清miR-24、X染色体连锁凋亡抑制蛋白(XIAP)mRNA表达情况,及其与患者预后的关系。方法选取2010年6月—2016年2月张家口市第一医院口腔ACC患者60例(口腔ACC组),以60名体检健康者作为正常对照组。收集所有研究对象的血清样本和ACC患者的癌组织、癌旁组织(距肿瘤组织边缘≥5 cm)样本,以及临床资料。检测血清和组织中miR-24、XIAP mRNA的相对表达量。采用Pearson相关分析评估miR-24与XIAP的相关性。对ACC患者进行随访。采用Kaplan-Meier生存曲线和Log-rankχ2检验分析血清miR-24、XIAP相对表达量与口腔ACC患者预后的关系。采用Cox回归分析评估影响口腔ACC患者预后的危险因素。结果与正常对照组比较,口腔ACC组血清miR-24相对表达量显著降低(P<0.05),XIAP mRNA相对表达量显著升高(P<0.05)。与癌旁组织比较,癌组织miR-24相对表达量显著降低(P<0.05),XIAP mRNA相对表达量显著升高(P<0.05)。口腔ACC患者血清miR-24与XIAP mRNA呈负相关(r=-0.486,P<0.05)。分别以口腔ACC患者血清miR-24和XIAP mRNA相对表达量的均值作为临界值,分为高表达组和低表达组。miR-24低表达组、XIAP mRNA高表达组神经侵袭和淋巴转移患者例数分别显著多于miR-24高表达组、XIAP mRNA低表达组(P<0.05)。Kaplan-Meier生存曲线分析结果显示,miR-24低表达组、XIAP mRNA高表达组累积生存率分别显著低于miR-24高表达组、XIAP mRNA低表达组(Log-rankχ2值分别为7.212、8.568,P值分别为0.007、0.003)。Cox回归分析结果显示,有神经侵袭、XIAP mRNA高表达是口腔ACC患者预后不良的独立危险因素(P<0.05),miR-24高表达是口腔ACC患者预后的独立保护因素(P<0.05)。结论口腔ACC患者血清miR-24和XIAP表达均与预后相关,或可作为新的口腔ACC预后评估生物标志物。
Objective To investigate the expression levels of serum microRNA-24(miR-24)and X-linked inhibitor of apoptosis protein(XIAP)mRNA in patients with oral adenoid cystic carcinoma(ACC)and the relationship with prognosis.Methods Totally,60 patients with oral ACC treated in Zhangjiakou First Hospital from June 2010 to February 2016 were enrolled as oral ACC group,and 60 healthy subjects during the same period were enrolled as healthy control group.Serum samples from all the subjects were collected.Cancer and para-cancer(≥5 cm from tumor tissue margin)tissue samples and clinical data were collected.The expression levels of miR-24 and XIAP mRNA in serum of the 2 groups were determined.The relationship between the expression levels of serum miR-24 and XIAP with the clinicopathologic characteristics of oral ACC patients were analyzed by pearson method.All the ACC patients were followed up.Kaplan-Meier survival curve and Log-rankχ2 test were used to analyze the relationship between the relative expression levels of serum miR-24 and XIAP mRNA and the prognosis of patients with oral ACC.Cox regression analysis was used to evaluate the prognostic risk factors of patients with oral ACC.Results Compared with healthy control group,the relative expression level of serum miR-24 in oral ACC group was decreased(P<0.05),and the relative expression level of XIAP mRNA was increased(P<0.05).Compared with para-cancer tissues,the relative expression of miR-24 in cancer tissues was decreased(P<0.05),and the relative expression of XIAP mRNA was increased(P<0.05).There was a negative correlation between serum miR-24 and XIAP mRNA in oral ACC patients(r=-0.486,P<0.05).Oral ACC patients were classified into high expression group and low expression group using the mean values of serum miR-24 and XIAP mRNA relative expression levels as cut-off values.The numbers of nerve invasion and lymphatic metastasis in miR-24 low expression group and XIAP mRNA high expression group were higher than those in miR-24 high expression group and XIAP mRNA low e
作者
周军
李立恒
张敏
ZHOU Jun;LI Liheng;ZHANG Min(Department of Stomatology,Zhangjiakou First Hospital,Zhangjiakou 075000,Hebei,China;Department of Stomatology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China;Clinical College of Zhangjiakou University,Zhangjiakou 075000,Hebei,China)
出处
《检验医学》
CAS
2023年第10期930-935,共6页
Laboratory Medicine
基金
张家口市科学技术和地震局资助项目(1821056D)。
