摘要
近年来,利川市黄连白绢病频发,严重影响黄连的产量和品质。为明确病原菌种类、生物学特性和有效杀菌剂,采用组织分离法分离病原菌,获得5株形态特征相似的菌株LC1~LC5,选择代表性菌株LC1进行致病性试验,结果符合柯赫氏法则。通过形态学分析与rDNA-ITS和rDNA-LSU序列的分子生物学分析,鉴定病原菌LC1为罗耳阿太菌(Athelia rolfsii)。其次对LC1的生物学特性进行分析,结果表明其最适生长温度为30℃,最适pH值为5~7,最适碳氮源分别为可溶性淀粉和牛肉浸膏。最后采用菌丝生长速率法进行室内毒力测定,筛选有效杀菌剂,结果表明,化学杀菌剂中,噻呋酰胺、咯菌腈、戊唑醇和吡唑醚菌酯的抑菌效果较好,EC_(50)值均小于1 mg·L^(-1);生物杀菌剂中,蛇床子素的抑菌效果较好,EC50值为14.25 mg·L^(-1),可作为备选药剂。该研究结果为利川市黄连白绢病的田间防控提供了一定的科学依据。
In recent years,southern blight of Coptis chinensis occurred frequently in Lichuan city,which se-riously affected the quality and yield of C.chinensis.This study aims to identify the pathogen,clarify the biological characteristics,and screen the effective fungicides.The tissue culture method was applied to isolate the pathogen and the results showed the five strains LC1~LC5 were obtained based on the similar morphological features,a representative LC1 strain was selected for the pathogenicity test and fulfilled the Koch's postulates.The pathogen LC1 was identified as Athelia rofsii by the morphology and combined sequence analysis of rDNA-ITS and rDNA-LSU.Moreover,the biological characteristics were analyzed that the optimum growth temperature for LC1 was 30℃ and pH ranged from 5 to 7.Soluble starch and beef extract were the best carbon and nitrogen sources respectively.Furthermore,mycelium growth rate method was used to determine the most effective fungicides.The results of laboratory virulence test showed that the EC_(50)of thifluzamide,fludioxonil,tebuconazole and tebuconazole in the chemical fungicide were less than 1 mg·L^(-1).Among the biological fungicide,osthole had a good antibacterial effect,with EC_(50)of 14.25 mg·L^(-1),which could be used as a candidate agent.The result provided a scientific basis for field control of southern blight of C.chinensis in Lichuan.
作者
王帆帆
唐涛
何瑜
郭晓亮
段媛媛
游景茂
WANG Fanfan;TANG Tao;HE Yu;GUO Xiaoliang;DUAN Yuanyuan;YOU Jingmao(Institute of Chinese Herbel Medicine,Hubei Academy of Agricultural Science,Key Laboratory for Biology and Cultivation of Chinese Medicinal Materials of Ministry of Agriculture and Rural Affairs,Hubei Standardized Production of Traditional Chinese Medicine(GAP)Engineering Research Center,Enshi 445000,China;Hubei Provincial Engineering Research Center of Under-forest Economy,Hubei Academy of Agricultural Science,Wuhan 430064,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2023年第5期789-795,共7页
Acta Phytopathologica Sinica
基金
湖北省农业科技创新中心重大科技研发项目(2020-620-000-002-04)
湖北省重点研发计划项目(2022BBA153)
国家现代农业产业技术体系建设专项(CARS-21)
2022中央引导地方科技发展资金项目(2022BGE256)。
关键词
黄连
白绢病
罗氏阿太菌
生物学特性
杀菌剂
Coptis chinensis
southern blight
Athelia rofsi
biological characteristics
fungicides
