摘要
本研究旨在探讨植物源性白桦脂醇(BET)与抗阻运动合并应用对高脂饮食(HFD)导致的大鼠认知障碍的改善作用及其潜在机制。通过使用HFD饲料建立大鼠认知障碍模型,分为七组实验:正常对照组(Con组)、HFD组、HFD+RE组、HFD+L-BET组、HFD+M-BET组、HFD+H-BET组和HFD+RE+H-BET组。其中,HFD+RE组及HFD+RE+H-BET组的大鼠进行了抗阻运动,而HFD+L/M/H-BET组则分别给予不同剂量的白桦脂醇处理。经过六周的干预,通过Morris水迷宫系统对大鼠的认知功能进行评估,同时检测海马神经元形态、氧化应激指标、炎症指标及关键蛋白和基因的表达水平。结果显示,HFD+RE+H-BET组相较于HFD+RE组和HFD+H-BET组,有明显的认知功能改善,海马神经元结构恢复、抗氧化指标提高、炎症水平降低,以及关键蛋白和基因的表达水平发生改变。这暗示白桦脂醇结合抗阻运动可以有效改善HFD导致的大鼠认知障碍,其作用机制可能与调节Nrf2介导的氧化应激反应、NF-κB介导的炎症反应和脂代谢基因的表达有关。
The objective of this study is to investigate the combined effects and potential mechanisms of betulin(BET) and resistance exercise on cognitive impairments induced by a high-fat diet(HFD) in rats.A rat model of cognitive impairment was established using HFD feed,divided into seven experimental groups:Normal control group(Con group),HFD group,HFD+RE group,HFD+L-BET group,HFD+M-BET group,HFD+H-BET group,and HFD+RE+H-BET group.Among them,rats in the HFD+RE group and HFD+RE+H-BET group underwent resistance exercise,while rats in the HFD +L/M/H-BET groups were treated with different doses of betulin.After six weeks of intervention,cognitive functions of the rats were evaluated through the Morris Water Maze system.Additionally,the morphology of hippocampal neurons,oxidative stress markers,inflammation markers,and the expression levels of key proteins and genes were examined.The results demonstrated that compared to the HFD +RE group and HFD+H-BET group,the HFD+RE+H-BET group exhibited significant improvements in cognitive functions,restoration of hippocampal neuron structures,increased anti-oxidative markers,reduced inflammation levels,and altered expression levels of key proteins and genes.This suggests that betulin combined with resistance exercise can effectively improve cognitive impairments caused by HFD.Its mechanism of action may be related to the modulation of Nrf2-mediated oxidative stress response,NF-κB mediated inflammatory response,and the expression of lipid metabolism genes.
作者
蒋靖柯
Jiang Jingke(Shanghai Industry and Commerce Foreign Languages College,Shanghai,201399)
出处
《分子植物育种》
CAS
北大核心
2023年第23期7892-7898,共7页
Molecular Plant Breeding
关键词
白桦脂醇
抗阻运动
高脂饮食
认知障碍
神经生物学
Betulin
Resistance exercise
High-fat diet
Cognitive impairment
Neurobiology
