摘要
目的了解台州市售婴幼儿谷类辅助食品中克罗诺杆菌的污染状况、分子分型特征和耐药性。方法参照GB 4789.40—2016对样品进行定性和定量检测,分离到的菌株进行脉冲场凝胶电泳(PFGE)分型和微量肉汤稀释法药敏试验。结果335份样品中分离到41株克罗诺杆菌,总检出率为12.2%;41份阳性样品定量结果为0.36~7.5 MPN/100 g;食用方式上烹煮类样品检出率最高(46.9%),购买途径上网购样品检出率最高(24.0%);41株菌株PFGE聚类分析被分为34个带型,相似度为45.1%~100.0%,从同一厂家不同种类样品和不同厂家样品中均有检出同一克隆株;菌株对头孢唑啉和呋喃妥因的敏感率仅为48.8%和39.0%,其中9株同时对这两种抗生素不敏感。结论台州市售婴幼儿谷类辅助食品存在克罗诺杆菌的污染,应关注烹煮类和网购类食品导致婴幼儿感染克罗诺杆菌的风险。
Objective To investigate the contamination,molecular typing characteristics,and drug resistance of Cronobacter in cereal-based infant food in Taizhou.Methods All samples were detected qualitatively and quantitatively according to GB 4789.40—2016.Isolated strains were typed using pulse field gel electrophoresis(PFGE),and antibiotic susceptibility tests were performed using the micro-broth dilution method.Results Forty-one strains were isolated from 335 samples.The total detection rate was 12.2%,and the quantitative results were 0.36-7.5 MPN/100 g.The cooked sample(46.9%)had the highest detection rate among edible samples,and the sample purchased online(24.0%)had the highest detection rate among purchased samples.Forty-one strains were divided into 34 PFGE patterns with similarity rates of 45.1%-100.0%,and some strains from different enterprises or different branches of one enterprise belonged to the same clone.Their insensitivity rates to cefazolin and nitrofurantoin were 48.8%and 39.0%,respectively,and 9 strains were insensitive to both.Conclusion Some cereal-based supplementary foods for infants were contaminated with Cronobacter in Taizhou.It is necessary to pay attention to the risk of Cronobacter infection in infants caused by cooked food and food purchased online.
作者
陈喜凯
盛莹
沈伟伟
裘丹红
CHEN Xikai;SHENG Ying;SHEN Weiwei;QIU Danhong(Taizhou Center for Disease Control and Prevention,Zhejiang Taizhou 318000,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2023年第7期1075-1079,共5页
Chinese Journal of Food Hygiene
基金
台州市科技局社会发展科技项目(20ywb106)。
关键词
谷类辅助食品
克罗诺杆菌
药敏性试验
脉冲场凝胶电泳
Cereal-based supplementary food
Cronobacter
antibiotic susceptibility test
pulsed field gel electrophoresis
