摘要
为分离能裂解鸭源大肠杆菌的噬菌体,采用富集法从鸭场污水中分离噬菌体,透射电镜观察其形态,结果显示,分离到1株噬菌体,电镜显示该噬菌体属长尾病毒科,命名为vB_EcoS214,头部直径约40 nm,尾部长约220 nm,宽约10 nm。该噬菌体有较宽的裂解谱,裂解力较强,可成为噬菌体鸡尾酒制剂的候选噬菌体。能裂解鸭源大肠杆菌噬菌体的成功分离,为鸭源大肠杆菌病的噬菌体疗法提供参考。
In order to isolate phages that can cleave Escherichia coli from duck,phages were isolated from duck farm sewage by enrichment method.Bacteriophage morphology and size were examined with a transmission electron microscope(TEM).The results showed that a phage was isolated.TEM showed that the phage belonged to the family Siphoviridae,named vB_EcoS214.The diameter of the head is about 40 nm,the length of the tail is about 220 nm and the width is about 10 nm.The phage has a wide cleavage spectrum and strong cleavage force,and it can be a candidate for cocktail preparation of phage.The successful isolation of phage that can cleave Escherichia coli from duck provide reference for phage therapy of duck colibacillosi.
作者
刘娜
陈红梅
江南松
黄瑜
傅光华
程龙飞
Liu Na;Chen Hongmei;Jiang Nansong;Huang Yu;Fu Guanghua;Cheng Longfei(Fujian Animal Diseases Control Technology Center/Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory for Avian Diseases Control and Prevention,Fuzhou350013)
出处
《福建畜牧兽医》
2023年第6期12-14,共3页
Fujian Journal of Animal Husbandry and Veterinary medicine
基金
财政部和农业农村部:国家现代农业产业技术体系项目(CARS-42)
农业科技创新联盟专项(CXLM202205)
福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021012)资助。
关键词
鸭
大肠杆菌
噬菌体
Duck
Escherichia coli
Phage
