摘要
目的:观察电针天枢、上巨虚对肠易激综合征(IBS)模型大鼠内脏敏感性、肠道运动功能的影响,并探讨电针干预治疗IBS的可能作用机制。方法:选用体质量(200±20)g的雄性清洁型Wistar大鼠61只,分为空白组(11只)和模型组(50只),模型组大鼠采用慢性和急性应激相结合方法连续干预10 d制备IBS模型;造模结束后,模型组大鼠通过腹壁撤回反射半定量评分结合应激后大便颗粒数与空白组相比具有统计学意义,以此筛选制备成功的模型,再随机分为模型组(10只)、天枢组(10只)和上巨虚组(10只);天枢组毫针直刺3 mm,上巨虚组毫针直刺5 mm,分别连接电针;模型组同电针组每天抓取并束缚固定20 min,不针刺;空白组不予以任何操作。干预结束后,各组大鼠在浅麻状态下,运用Powerlab数据采集分析系统,记录不同扩张压力下诱发腹外斜肌放电所产生相对应的结肠痛敏阈值;采用酶联免疫吸附法(ELISA)检测各组大鼠血清中SP含量以及免疫蛋白印迹(Western blot)方法检测远端结肠中SP-神经激肽1型受体(NK-1R)蛋白的表达。结果:与空白组相比,模型组大鼠结肠痛敏阈值明显降低,血清SP含量以及远端结肠NK-1R受体表达均明显升高,差异具有统计学意义(P<0.05);与模型组相比,电针干预治疗后结肠痛敏阈值明显升高(P<0.01),血清SP含量以及远端结肠NK-1R受体表达均明显降低,差异具有统计学意义(P<0.01或P<0.05)。结论:电针天枢、上巨虚可以通过降低血清SP含量和结肠SP受体NK-1R表达,降低IBS大鼠内脏敏感性,升高痛敏阈值,调节肠道运动功能。
Objective:To observe the effects of electro-needling Tianshu(ST25)and Shangjuxu(ST37)on visceral sensitivity and intestinal motility in IBS model rats,and to explore its possible action mechanism in treatment of irritable bowel syndrome(IBS).Methods:61 male Wistar rats(200±20 g)were randomly divided into the blank group(n=11)and the modeling group(n=50).The rats in the modeling group were continuously intervened for 10 days by the combination of chronic and acute stress to prepare IBS model.After the modeling,the rats in the modeling group were screened for successful models by the abdominal withdrawal reflex(AWR)semi-quantitative score and the number of stool particles after stress compared with those in the blank group.The successful models were subdivided into the model group(n=10),the Tianshu group(n=10)and the Shangjuxu group(n=10).The Tianshu group was punctured 3 mm straight with filiform needles,and the Shangjuxu group was directly punctured 5 mm with filiform needles,then both groups were connected to electro-acupuncture(EA).The rats in the model group were grasped and restrained for 20 minutes every day without acupuncture;the blank group was not given any intervention.After the intervention,the rats in each group were under light anesthesia,and the Powerlab data acquisition and analysis system was used to record the corresponding colonic pain sensitivity threshold induced by the discharge of the external oblique muscle under different expansion pressures.ELISA method was applied to detect the serum content of SP,and the Western Blot method was used to detect the protein expression of NK-1R in distal colon.Results:Compared with those in the blank group,the colonic pain sensitivity threshold was significantly decreased,the serum content of SP and the expression of NK-1R in the distal colon were significantly increased in the model group(P<0.01).Compared with those in the model group,the colonic pain sensitivity threshold were significantly increased(P<0.01),and the serum content of SP and the expression
作者
刘婷婷
陈少宗
赵香顺
石运来
于燕南
杨添淞
LIU Tingting;CHEN Shaozong;ZHAO Xiangshun;SHI Yunlai;YU Yannan;YANG Tiansong(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Institute of Acupuncture and Moxibustion,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong University of Traditional Chinese Medicine,Jinan 250355,China;The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Heilongjiang Key Laboratory of Traditional Chinese Medicine Informatics,Harbin 150040,China)
出处
《针灸临床杂志》
2023年第11期61-65,共5页
Journal of Clinical Acupuncture and Moxibustion
基金
国家重点研发计划,编号:2019YFC1712105
国家自然科学基金面上项目,编号:82074539。
关键词
肠易激综合征
电针
内脏敏感性
肠道运动功能
P物质
Irritable bowel syndrome
Electro-acupuncture
Visceral sensitivity
Intestinal motor function
Substance P
