摘要
目的分析血清腺苷脱氨酶(ADA)、白细胞介素-10(IL-10)及Toll样受体9(TLR9)与EB病毒(EBV)相关传染性单核细胞增多症(IM)患儿EBV-DNA载量的相关性。方法前瞻性选择2021年2月至2022年12月就诊于该院的98例EBV相关IM患儿作为IM组,另选同期于该院体检的50例健康儿童作为对照组。比较对照组、IM组血清ADA、IL-10及TLR9水平,线性回归分析ADA、IL-10、TLR9水平与IM发生的关系,绘制受试者工作特征(ROC)曲线分析血清ADA、IL-10、TLR9水平单独及联合预测IM发生的价值。根据EBV-DNA载量不同将IM组患儿分为低载量组(18例)、低-中载量组(25例)、中-高载量组(49例)、高载量组(6例)4个亚组,比较各组血清ADA、IL-10及TLR9水平,Spearman等级相关分析血清ADA、IL-10、TLR9水平与IM患儿EBV-DNA载量的关系。结果与对照组比较,IM组血清ADA、IL-10、TLR9水平升高(P<0.05);线性回归分析结果显示,ADA、IL-10、TLR9水平均与EBV相关IM的发生有关(P<0.05);ROC曲线结果发现血清ADA、IL-10、TLR9水平单独及联合预测EBV相关IM发生的曲线下面积分别为0.850(95%CI 0.791~0.910)、0.867(95%CI 0.811~0.924)、0.891(95%CI 0.840~0.943)、0.912(95%CI 0.843~0.956);血清ADA、IL-10、TLR9水平及EBV-DNA载量从高到低为高载量组、中-高载量组、低-中载量组、低载量组(P<0.05);Spearman等级相关结果显示,血清ADA(r=0.526,P<0.001)、IL-10(r=0.610,P<0.001)及TLR9(r=0.715,P<0.001)水平与EBV相关IM患儿EBV-DNA载量呈正相关。结论EBV相关IM患儿血清ADA、IL-10及TLR9水平异常升高,上述指标水平与EBV-DNA载量密切相关,且三者联合可有效预测EBV相关IM的发生。
Objective To study the correlation of serum adenosine deaminase(ADA),interleukin-10(IL-10)and Toll-like receptor 9(TLR9)with Epstein-Barr virus(EBV)-DNA load in children with EBV-associated infectious mononucleosis(IM).Methods A total of 98 children with EBV-associated IM who were admitted to the hospital from February 2021 to December 2022 were prospectively selected as the IM group,and 50 healthy children who underwent physical examination in the hospital during the same period were selected as the control group.The levels of ADA,IL-10 and TLR9 in the control group and IM group were compared,and the relationship between ADA,IL-10,TLR9 and IM was analyzed by linear regression.Receiver operating characteristic(ROC)curve was drawn to analyze the value of serum ADA,IL-10 and TLR9 levels and their combination in predicting IM.According to EBV-DNA load,children with IM were divided into four subgroups:low load group(18 cases),low-medium load group(25 cases),moderate-high load group(49 cases)and high load group(6 cases).The serum ADA,IL-10 and TLR9 levels were compared among the groups.Spearman rank correlation analysis was used to analyze the relationship between the serum ADA,IL-10,TLR9 levels and EBV-DNA load in children with IM.Results Compared with the control group,the levels of ADA,IL-10 and TLR9 were significantly increased in the IM group(P<0.05).Linear regression analysis showed that ADA,IL-10 and TLR9 levels were related to the occurrence of EBV-associated IM(P<0.05).The area under the curve(AUC)of ADA,IL-10,TLR9 and their combination were 0.850(95%CI 0.791-0.910),0.867(95%CI 0.811-0.924),0.891(0.840-0.943),and 0.912(95%CI 0.843-0.956),respectively.The levels of ADA,IL-10,TLR9 and EBV-DNA load from high to low were as follows:high load group,mediumhigh load group,low-medium load group and low load group(P<0.05).Spearman rank correlation analysis showed that serum ADA(r=0.526,P<0.001),IL-10(r=0.610,P<0.001)and TLR9(r=0.715,P<0.001)levels were positively correlated with EBV-DNA load in children with EBV-assoc
作者
李萍
安莉莉
韩冰
LI Ping;AN Lili;HAN Bing(Department of Pediatrics,Rizhao Maternal and Child Health Hospital,Rizhao,Shandong 276800,China)
出处
《国际检验医学杂志》
CAS
2023年第23期2924-2928,共5页
International Journal of Laboratory Medicine
基金
山东省优秀中青年科学家科研奖励基金(BS2020SW1444)。
