摘要
目的:探讨非小细胞肺癌(NSCLC)患者血清miR-146a与肿瘤坏死因子α(TNF-α)、T调节细胞(Treg)的相关性及miR-146a对患者预后的预测价值。方法:选择2020年6月—2021年11月在沧州市中心医院治疗的中晚期NSCLC患者162例。采用荧光定量PCR法检测患者血清中miR-146a的相对表达水平,采用酶联免疫法检测患者血清中TNF-α浓度,采用流式细胞术检测患者静脉血中的Treg细胞比例。采用Pearson相关分析miR-146a相对表达水平与TNF-α浓度、Treg细胞比例的相关性,采用多因素logistic回归分析miR-146a相对表达水平是否为NSCLC患者的预后危险因素,采用ROC曲线检测miR-146a对患者预后的预测效能。结果:NSCLC患者血清中miR-146a的相对表达水平为1.48±0.18,TNF-α的浓度为(29.46±4.19)μg/L,静脉血中Treg细胞比例为(4.03±0.85)%,且miR-146a相对表达水平与TNF-α浓度呈正相关(r=0.651,P<0.01),与Treg比例呈负相关(r=-0.676,P<0.01)。miR-146a相对表达水平和TNF-α浓度为预后危险因素(OR分别为1.95和1.39,均为P<0.01),Treg细胞比例为NSCLC患者死亡的保护因素(OR=0.53;P<0.01)。miR-146a相对表达水平的AUC为0.959,95%CI(0.934,0.985),P<0.01。结论:NSCLC患者的血清miR-146a相对表达水平与TNF-α浓度呈正相关,与Treg细胞比例呈负相关,是NSCLC患者死亡的危险因素,对NSCLC预后有较高的预测价值。
OBJECTIVE:To investigate correlations of serum miR-146a with tumor necrosis factorα(TNF-α)and T-regulatory cells(Treg)in patients with non-small cell lung cancer(NSCLC)and prognostic valuesa in the patients.METHODS:From June 2020 to November 2021,a total of 162 patients with advanced NSCLC who were treated in Cangzhou Central Hospital were selected for our study.Levels of miR-146a in patients were detected using fluorescence quantitative PCR ELISA,serum levels of miR-146a and TNF-αwere detected using enzyme-linked immunoassay,and Treg levels were detected using flow cytometry.Pearson correlation was used to analyze correlations among miR-146a expressions,TNF-αconcentrations and Treg proportion.Multi-factor logistic regression was used to analyze whether the relative expression levels of miR-146a was a prognostic risk factor for the patients.ROC curve was used to compare the predictive efficacy of miR-146a on prognosis.RESULTS:The relative expression levels of miR-146a was positively correlated with the concentrations of TNF-α(r=0.651,P<0.01),and negatively correlated with the proportions of Treg(r=-0.676,P<0.01).The relative expression levels of miR-146a and the concentrations of TNF-αwere prognostic risk factors(OR=1.95 and 1.39,respectively;P<0.01).Treg was a prognostic protective factor for death in NSCLC patients(OR=0.53;P<0.01).The AUC of miR-146a was 0.959 and 95%CI(0.934,0.985),P<0.01.CONCLUSION:Serum miR-146a in NSCLC patients was positively correlated with TNF-αand negatively correlated with Treg,which was shown to be a risk factor for death in NSCLC patients and to have high prognostic value for NSCLC.
作者
许金花
李静静
吴国峰
任亚俊
王雪
张骞云
XU Jinhua;LI Jingjing;WU Guofeng;REN Yajun;WANG Xue;ZHANG Qianyun(The Second Department of Respiratory and Critical Care Medicine,Cangzhou Central Hospital,Cangzhou 061014;The Second Department of Anesthesia,Cangzhou Central Hospital,Cangzhou 061014,Hebei,China)
出处
《癌变．畸变．突变》
CAS
2023年第6期462-466,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
沧州市科学技术局(204106114)。
