摘要
该文研究了高压热杀菌处理(high-pressure thermal sterilization,HPTS)结合溶菌酶对枯草杆菌芽孢的灭活动力学,并探讨了芽孢灭活量与芽孢悬浮液OD_(600)、OD_(260)、OD_(280)值之间的相关性。分别用一级动力学模型和Weibull模型描述HPTS结合溶菌酶灭活芽孢的动力学,并对动力学模型拟合度进行比较分析,以OD_(600)值表征芽孢内容物的释放量,OD_(260)、OD_(280)值表征芽孢核酸及蛋白的泄漏量。在600 MPa下,温度为65、75℃,溶菌酶质量分数为0.05%、0.10%、0.30%时,随着温度和溶菌酶浓度的提高,芽孢灭活量不断增大。Weibull模型的平均决定系数R~2=0.972,精确因子A_(f)为1.05~1.11,偏差因子B_(f)为1.02~1.04,均方根误差为0.14~0.48,Weibull模型的拟合效果优于一级动力学模型。随着处理时间的延长,芽孢悬浮液OD_(600)、OD_(260)、OD_(280)值也不断变化,与芽孢灭活量变化的相关系数分别为-0.972、0.828、0.848,芽孢灭活量与芽孢悬浮液OD_(600)、OD_(260)、OD_(280)值都呈极显著相关但芽孢悬浮液OD_(600)值与芽孢灭活量之间的相关性更显著且OD_(600)值简单易测,可用于快速准确地预测芽孢灭活效果。
In this paper,the inactivation kinetics of Bacillus subtilis spores by high-pressure thermal sterilization(HPTS) combining with lysozyme was studied,and the correlation between the inactivation amount of spores and the OD values of spore suspensions was investigated.The first-order kinetic model and Weibull model were used to describe the inactivation kinetics of the spores by HPTS combining with lysozyme,and the fitting effects of the kinetic models were compared and analyzed.The OD_(600) value was used to characterize the release of spore content.The OD_(260) and OD_(280) values were used to characterize the leakage of spore′s nucleic acids and proteins.At 600 MPa,65 ℃ and 75 ℃,with 0.05%,0.10%,and 0.30% lysozyme concentration,the inactivation of spores increased continuously with the increase of temperature and lysozyme concentration.The average coefficient of determination R~2 was 0.972 for the Weibull model,the accuracy factor A_(f) was 1.05-1.11,the bias factor B_(f) was 1.02-1.04,and the root mean square error RMSE was 0.14-0.48.The fitting effects of the Weibull model were better than the first-order kinetic model.With the increase of the treatment time,the OD_(600),OD_(260),OD_(280 )values of the spore suspension also changed continuously,and their correlation coefficients with the changes of the spore′s inactivation amount were-0.972,0.828,0.848 respectively.The amount of spore inactivation was extremely significantly correlated with the OD_(600),OD_(260),and OD_(280) values of the spore suspension.However,the correlation between the OD_(600) value of the spore suspension and the amount of spore inactivation the most significant,and the OD_(600) value was easy to measure and could be used to predict the inactivation effect of spores quickly and reliably.
作者
王旭娟
刘月
李佳佳
武思睿
王传发
辛伟山
章中
WANG Xujuan;LIU Yue;LI Jiajia;WU Sirui;WANG Chuanfa;XIN Weishan;ZHANG Zhong(School of Food&Wine,Ningxia University,Yinchuan 750021,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第22期57-63,共7页
Food and Fermentation Industries
基金
国家自然科学基金项目(31760474,31460410)。
关键词
芽孢
高压热杀菌处理
溶菌酶
灭活动力学
相关性分析
spore
high-pressure thermal sterilization(HPTS)
lysozyme
inactivation kinetics
correlation analysis
