摘要
基于三维荧光光谱技术结合荧光区域积分法,研究了岩门秀芽在不同冷泡时间下浸出液样品的三维荧光光谱特征。通过激发扫描获取激发-发射矩阵光谱,识别荧光峰的位置并采用荧光区域积分法计算荧光峰所处区域内荧光强度的积分体积值,以探究岩门秀芽主要浸出物种类及其动态变化规律。研究结果显示,在不同浸泡时间下,岩门秀芽浸出液的三维荧光光谱都会产生明显的瑞利散射和红移现象,其主要浸出物:黄酮及黄酮醇类物质、儿茶素和叶绿素a的含量也在不断地变化。在20 min时,茶叶功效成分的含量保留较高且儿茶素含量较低,为冷泡的最佳饮用时间。该研究为实现基于三维荧光光谱技术快速检测茶叶主要成分和鉴别茶叶种类奠定了基础,也为茶叶的科学浸泡和饮用提供参考。
Based on three-dimensional fluorescence excitation-emission matrix spectroscopy combined with the fluorescence regional integration,the spectral characteristics of the leaching samples of cold-brewing Yanmen Xiuya tea under different soaking times were studied.The excitation-emission matrix spectra were obtained through excitation scanning,and on this basis,the position of the fluorescent peak was identified and the integrated volume value of the fluorescence intensity in the area where the fluorescence peak was located was calculated by the fluorescence regional integration to explore the main extract types and dynamic changes of the Yanmen Xiuya tea.The results of the study show that the three-dimensional fluorescence excitation-emission matrix spectroscopy of the Yanmen Xiuya leaching liquid produces obvious Rayleigh scattering and red-shifting under different soaking times,and the content of the main extracts:flavonoids and flavonols,catechins and chlorophyll a is also constantly changing.At the 20-minute interval,the content of tea retains a large amount of functional ingredients and a low amount of catechin,which is the best drinking time for cold brewing.This study will provide the foundation for rapid detection of the main components of tea and the identification of tea types based on three-dimensional fluorescence excitation-emission matrix spectroscopy and also give suggestions for scientific soaking and drinking of tea.
作者
黄玉双
于秀静
吴蝶
谭超
HUANG Yushuang;YU Xiujing;WU Die;TAN Chao(Key Lab of Process Analysis and Control of Sichuan Universities,Yibin University,Yibin 644000,Sichuan,China)
出处
《化学研究》
CAS
2023年第6期490-495,共6页
Chemical Research
基金
2022年国家级大学生创新创业训练计划项目(202210641029)
宜宾学院本科工程项目(JGZ202211、XGK202114)。
关键词
三维荧光
荧光区域积分法
瑞利散射
红移
冷泡茶
three-dimensional fluorescence
fluorescence regional integration
Rayleigh scattering
red-shifted
cold-brewing tea
