摘要
为深入探究丝素蛋白载药纳米粒的制备机制与方法,开发智能响应型丝素载药纳米粒,综述了丝素蛋白的微观结构与性能特点,概述了采用沉淀法、盐析法、电喷雾法、乳液法和脱溶剂法制备丝素蛋白纳米粒的机制与技术特点,分析了丝素蛋白载药方法与药物缓控释实现途径,并重点介绍了pH值响应和磁响应2种靶向释药的机制、方法及其应用。提出:可通过控制纳米粒大小、形状和表面电荷、与药物的化学键结合等方法来提高药物利用效率;通过改变丝素SilkⅠ和SilkⅡ结晶结构的含量来改变丝素的降解速度,或者通过丝素蛋白与其它不同降解速度的高聚物复合制备纳米粒从而实现药物的可控释放。此外,还可将多种智能响应结合来提高响应效率,最大程度减小药物的全身性毒副作用,实现靶向释药与精准医疗。
Significance Because of their unique size effect,drug-loaded nanoparticles can protect drugs from being cleared by the liver and spleen,break through the physiological barrier of the human body,act directly on cells and tissues,provide local tissues with continuous high blood concentrations,enhance cell infiltration and reduce the toxicity risk of patients′normal cells.Silk fibroin(SF)has attracted much attention as a nanodrug delivery carrier material because of its excellent biocompatibility,biodegradability,low immunogenicity,high binding ability to various drugs and mild preparation conditions.In particular,SF has different functional groups,which can be chemically modified or surface modified in a variety of ways to improve the drug loading rate,trigger biological reactions to cells through covalent binding with targeted ligands,achieve targeted drug release,and improve the therapeutic efficiency.Therefore,SF is a very prospective protein as a drug-loaded base material.This paper analyzed the pelletizing principles of SF as a drug carrier material and various ways of preparing them and highlighted the mechanism of the sustained and controlled drug release of SF nanoparticles,especially introducing the smart drug release responses of SF nanoparticles to pH changes or magnetic fields,which provided a useful reference for the preparation and application of SF in drug carrier materials.Progress SF as a drug carrier is mainly achieved by inducing or modifying the SilkⅠstructure to the SilkⅡstructure.The current research mainly focuses on three aspects.The first is to explore the role and mechanism of the physical and chemical properties of SF in drug-loaded nanoparticles.SF has two different secondary structures,SilkⅠand SilkⅡ.By solvent treatment,such as ethanol,or under the action of high heat and high shear force,SilkⅠtransforms to SilkⅡ,leading to the self-assembly of SF,thus forming micro/nanospheres.The second is to study the preparation methods of SF drug-loaded nanoparticles to improve their
作者
张子凡
李鹏飞
王建南
许建梅
ZHANG Zifan;LI Pengfei;WANG Jiannan;XU Jianmei(College of Textile and Clothing Engineering,Soochow University,Suzhou,Jiangsu215021,China;Key Laboratory of Textile Industry for Silk Products in Medical and Health Use,Soochow University,Suzhou,Jiangsu215127,China)
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2023年第10期205-213,共9页
Journal of Textile Research
基金
纺织行业医疗健康用蚕丝制品重点实验室基金项目(Q811580321)。
关键词
丝素蛋白
纳米粒子
载药递送
智能响应
靶向释药
silk fibroin
nanoparticle
drug delivery
intelligent response
targeted drug release
