摘要
为了研究中草药复合添加剂对工作犬血清生化指标、抗氧化能力和免疫功能的影响,试验将18只拉布拉多工作犬随机分成3组,分别为对照组、试验A组和试验B组,每组3个重复,每个重复2只(公母各1只),试验期共50 d。试验第1~7天,各组均饲喂基础日粮;第8~50天,对照组饲喂基础日粮,试验A组饲喂基础日粮+5 g/kg(按日粮重计)的中草药复合添加剂(由黄芪、党参、茯苓、蒲公英、金银花、淫羊藿配制而成),试验B组饲喂基础日粮+10 g/kg(按日粮重计)的中草药复合添加剂。试验结束后,前臂静脉采血,测定各组血清生化指标[总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)质量浓度、谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、肌酸激酶(CK)活性]、抗氧化指标[过氧化氢酶(CAT)、总超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)浓度]和免疫指标[免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、免疫球蛋白M(IgM)质量浓度]。结果表明:试验A组血清GLB质量浓度显著高于对照组(P<0.05),试验B组血清TP、GLB质量浓度显著高于对照组(P<0.05);试验A组和试验B组血清CK活性极显著低于对照组(P<0.01),其他血清生化指标与对照组均差异不显著(P>0.05);试验A组和试验B组所有血清生化指标均差异不显著(P>0.05)。试验B组血清CAT、SOD和GSH-Px活性显著或极显著高于对照组和试验A组(P<0.05或P<0.01),试验A组血清CAT和SOD活性显著高于对照组(P<0.05),试验A组、B组血清MDA浓度极显著低于对照组(P<0.01)。试验B组血清IgA、IgG和IgM质量浓度显著或极显著高于对照组和试验A组(P<0.05或P<0.01);而试验A组仅血清IgG质量浓度显著高于对照组(P<0.05),IgA和IgM质量浓度与对照组差异不显著(P>0.05)。说明日粮中添加中草药复合添加剂能提高工作犬抗氧化能力和免疫功能,且不会对肝脏和肾脏产生损伤,其中添加剂量�
In order to study the effects of Chinese herbal compound additives on the serum biochemical indexes,antioxidant capacity and immune function of working dogs,18 Labrador Retriever were randomly divided into three groups:control group,test group A and test group B,with three replicates in each group,two for each replicate(one for male and one for female).The experiment lasted for 50 days.From the Ist to 7th days of the experiment,the animals in each group were fed with a basal diet.From the 8th day to the 5Oth day,the animals in the control group were fed with the basal diet,and the animals in the test group A were fed with the basal diet+5 g/kg(according to the diet weight)of Chinese herbal compound additives(prepared by Astragalus,Codonopsis pilosula,Poria cocos,Taraxacum,Lonicera japonica and Epimedium),and the animals in the test group B was fed with the basal diet+10 g/kg(according to the diet weight)of Chinese herbal compound additives.After the test,blood was collected from forearm vein,and serum biochemical indexes(total protein[TP],albumin[ALB],globulin[CLB]mass concentration,alanine transaminase[ALT],aspartate transaminase[AST],alkaline phosphatase[ALP],lactate dehydrogenase[LDH],creatine kinase[CK]activity),antioxidant indexes(Catalase[CAT],total superoxide dismutase[SOD],and glutathione peroxidase[GSH-Px]activities and malondialdehyde[MDA]concentration)and immune indexes(immunoglobulin A[IgA],immunoglobulin G[IgG],immunoglobulin M[IgM]mass concentration)of each group were determined.The results showed that the serum GLB mass concentration in test group A was significantly higher than that in the control group(P<0.05),while the serum TP and GLB mass concentrations in test group B were significantly higher than those in the control group(P<0.05).The serum CK activity in test group A and test group B was significantly lower than that in the control group(P<0.01),and there was no significant difference in other serum biochemical indicators between the test groups and the control group(P>0.05).There was no s
作者
王宝杰
姜同泉
肖发沂
齐艳君
王福江
朱绍辉
WANG Baojie;JIANG Tongquan;XIAO Fayi;QI Yanjun;WANG Fujiang;ZHU Shaohui(Shandong Vocational Animal Science and Veterinary College,Weifang 261061,China;Weifang Institute of Technology,Weifang 261101,China;Weifang Animal Husbandry Development Center,Weifang 261011,China)
出处
《黑龙江畜牧兽医》
北大核心
2023年第22期136-140,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
潍坊市科技发展计划项目(2022GX025)。
关键词
中草药复合添加剂
工作犬
血清生化指标
抗氧化能力
免疫功能
Chinese herbal compound additives
working dogs
serum biochemical indexes
antioxidant capacity
immune function
