摘要
研究采用核磁共振碳谱法(^(13)C NMR),首先识别食用油脂中甘油一酯(MG)、甘油二酯(DG)、甘油三酯(TG)及游离脂肪酸(FFA)等组分的特征峰,然后确定特征峰响应值与组分含量的关系,最后建立了食用油脂中甘油酯组成的核磁共振碳谱分析方法。实验结果表明,样品量为200 mg食用油甘油酯,溶剂为0.5 mL氘代氯仿,^(13)CNMR谱采集条件为脉冲序列zgpg30,扫描次数(NS)为100,脉冲宽度(P1)为30μs,能够快速的测定甘油酯样品中MG、DG、TG和FFA的相对质量分数,且所测定结果与柱层析分离法测定的结果没有显著差异。该方法对响应值最低的游离脂肪酸的检出限可低至1.35%,定量限可低至4.45%。
The characteristic peak of monoglyceride(MG),diacylglyceride(DG),triglycerides(TG) and free fatty acid(FFA) from edible oils and fats was identified by ^(13)C NMR.The relationship between characteristic peak response value and component content was determined.A method for the determination of glyceride composition in edible oil by nuclear magnetic resonance carbon spectroscopy was established.The experimental results indicated that the relative mass percentage contents of MG,DG,TG and FFA in samples can be determined rapidly when the test conditions were 200 mg sample,0.5 mL deuterated chloroform,^(13)C NMR spectrum acquisition pulse sequence ZGPG30,scanning times(NS) 100,pulse width(P1) 30 μs.There was no significant difference between the measured results and those determined by column chromatography.The detection limit and quantification limit of free fatty acids with the lowest response value could be as low as 1.35% and 4.45%,respectively.
作者
肖新生
谢依琳
刘芳
蒋黎艳
Xiao Xinsheng;Xie Yilin;Liu Fang;Jiang Liyan(Department of Chemistry and Biological Engineering,Hunan University of Science and Engineering,Yongzhou 425199)
出处
《中国粮油学报》
CSCD
北大核心
2023年第9期210-218,共9页
Journal of the Chinese Cereals and Oils Association
基金
湖南省自然科学基金面上项目(2021JJ30289)
永州市2019年指导性科技计划项目(永科发[2019]15号,2019-yzkj-02)。
关键词
核磁共振光谱法
甘油酯
食用油
^(13)C
nuclear magnetic resonance spectroscopy
glyceride
edible oil and fat
^(13)C
