摘要
为探讨盐碱胁迫下产ACC脱氨酶促生菌对绿豆叶片脂质代谢的影响,采用液相色谱质谱联用技术分析盐碱胁迫下接种产ACC脱氨酶促生菌(DQJC1)后绿豆叶片内脂类代谢产物的变化。结果表明:盐碱胁迫下接种DQJC1可以促进绿豆生长,显著提高了绿豆植株的株高(34.87%)、地上部鲜质量和干质量(44.07%和46.43%)、地下部鲜质量和干质量(30.56%和23.68%)及叶绿素含量(41.61%)。同时在绿豆叶片中共筛选到61个具有显著性差异的代谢产物,其中包括32种甘油磷脂类、14种固醇类、4种甘油糖脂类、3种鞘脂类和8种其他脂类;其中上调的代谢物主要为甘油磷脂(PC、PG、LPC、PI、PE、LPG)、甘油糖脂(SQDG47∶11)及鞘脂(CerG1d18∶2/16∶0+O),下调的代谢物主要为固醇(TG),甘油糖脂(SQDG:16∶0/16∶0)及鞘脂(CerG1d34∶2+O)。KEGG通路富集分析发现筛选出的差异代谢物中有6种代谢物被富集到9条代谢通路中,包括甘油磷脂代谢通路、甘油脂代谢通路、自噬性溶酶体代谢通路、糖基磷脂酰肌醇代谢通路、醚脂质代谢通路、亚油酸代谢通路、磷脂酰肌醇代谢通路、α-亚油酸代谢通路和花生四烯酸代谢通路。
To investigate the effects of ACC deaminase bacteria under saline stress on the lipid metabolism of mung bean leaves,liquid chromatography mass spectrometry was used to analyze the changes of lipid metabolites in the leaves of ACC(DQJC1).It was found that DQJC1 inoculation under saline stress promoted the growth of mung bean,which significantly increased the plant height(34.87%),aboveground parts fresh weight(44.07%and 46.43%),underground partsdry weightof mung bean plants(30.56%and 23.68%)in the chlorophyll content(41.61%).At the same time,61 metabolites with significant differences(P<0.05)were selected,including 32 glycerophospholipids,14 sterols,4 glycerol lipids,3 sphingolipids and 8 other lipids.The upregulated metabolites were glycerol phospholipids(PC,PG,LPC,PI,PE,LPG),glycerol lipids(SQDG47∶11)and sphingolipids(CerG1d18∶2/16∶0+O).The upregulated metabolites were mainly sterols(TG);glycerol glucolipids(SQDG∶16∶0/16∶0)and sphingolipids(CerG1d34∶2+O).KEGG pathway enrichment analysis found that six of the selected differential metabolites were enriched in 9 metabolic pathways,including glycerophospholipid metabolism pathway,glycerol lipid metabolismpathway,autophagy-other pathway,glycosylphosphatidylinositol(GPI)-anchor biosynthesispathway,ether lipid metabolismpathway,linoleic acid metabolismpathway,phosphatidylinositol signaling systempathway,alpha-linolenic acid metabolismpathway and arachidonic acid metabolism pathway.
作者
李鑫
张美珍
郑翘楚
刘权
黄玉兰
殷奎德
LI Xin;ZHANG Meizhen;ZHENG Qiaochu;LIU Quan;HUANG Yulan;YIN Kuide(College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China)
出处
《干旱地区农业研究》
CSCD
北大核心
2023年第6期72-78,87,共8页
Agricultural Research in the Arid Areas
基金
黑龙江八一农垦大学研究生创新科研项目(YJSCX2021-Y61)
黑龙江省自然科学基金项目(LH2021C064)。
关键词
绿豆幼苗
盐碱胁迫
产ACC脱氨酶促生菌
脂质代谢
mung bean seedling
salt-salinity stress
producing ACC deaminase promoting bacteria
lipid metabolism
