摘要
目的 探讨Duffy血型不同表型对红细胞储存及清除趋化因子的影响。方法 收集24份红细胞标本,用抗人球蛋白试验法检测Duffy血型表型,使用ELISA法检测红细胞CCL2、CCL5、CXCL8以及CCL11含量及其趋化因子清除功能。使用流式分析仪检测红细胞上Duffy抗原的表达。结果 Duffy血型Fy(a+b-)与Fy(a+b+)表型的红细胞裂解液CCL2含量(41.1±14.7) pg/mL vs(63.1±20.8)pg/mL有差异(P<0.05),而CCL5(794.5±320.1)pg/mL vs(846.9±359.4)pg/mL、CXCL8(59.5±34.2)pg/mL vs(49.1±11.9)pg/mL及CCL11的含量(109.1±25.1)pg/mL vs(158.6±56.0)pg/mL均无差异(P>0.05)。Duffy血型Fy(a+b-)与Fy(a+b+)表型对于CCL2(1 471±202.1)pg/mL vs(1 860±267.5)pg/mL及CCL5清除功能(848.5±461.7)pg/mL vs(1 797±546.1)pg/mL均有差异(P<0.05),而对于CXCL8(1 851±180.7)pg/mL vs(1862±248.3)pg/mL及CCL11(691.0±125.7)pg/mL vs(781.7±293.8)pg/mL的清除功能无差异(P>0.05)。Duffy血型Fy(a+b-)与Fy(a+b+)表型的红细胞上的Duffy抗原表达平均荧光强度:(105.3±20.45)vs(111.9±18.30)无差异(P>0.05)。结论 Duffy血型Fy(a+b+)和Fy(a+b-)表型对红细胞储存及清除趋化因子的影响存在差异,Fy(a+b+)表型要比Fy(a+b-)表型红细胞能够储存更多的趋化因子以及具有更强的趋化因子清除功能。
Objective To investigate the effect of phenotypes of Duffy blood group on chemokine storage and chemokine scavenging function of erythrocytes.Methods Twenty-four erythrocyte samples were collected and tested Duffy blood phenotype using the anti-human globulin method,and erythrocyte CCL2,CCL5,CXCL8,and CCL11 content and their chemokine scavenging function using ELISA.The expression of Duffy antigens on erythrocytes was detected using a flow analyzer.Results The difference in CCL2 content(41.1±14.7 pg/mL vs 63.1±20.8 pg/mL)of erythrocyte lysate between Fy(a+b-)and Fy(a+b+)phenotype was statistically significant(P<0.05),however,the difference in the content of CCL5(794.5±320.1 pg/mL vs 846.9±359.4 pg/mL),CXCL8(59.5±34.2 pg/mL vs 49.1±11.9 pg/mL),and CCL11(109.1±25.1 pg/mL vs 158.6±56.0 pg/mL)were not statistically significant(P>0.05).The difference in the scavenging function of CCL2(1471±202.1 pg/mL vs 1860±267.5 pg/mL)and CCL5(848.5±461.7 pg/mL vs 1797±546.1pg/mL)between Fy(a+b-)and Fy(a+b+)phenotype were statistically significant(P<0.05),however,for CXCL8(1851±180.7 pg/mL vs 1862±248.3 pg/mL)and CCL11(691.0±125.7 pg/mL vs 781.7±293.8 pg/mL)scavenging function the difference were not statistically significant(P>0.05).The difference in Duffy antigen expression(mean fluorescent intensity:105.3±20.45 vs 111.9±18.30)on erythrocytes between Fy(a+b-)and Fy(a+b+)phenotype was not statistically significant(P>0.05).Conclusion The Fy(a+b+)and Fy(a+b-)phenotypes of the Duffy blood group can affect the chemokine storage and scavenging function of erythrocytes.Fy(a+b+)phenotypes are able to store more chemokines and have a stronger chemokine scavenging function than Fy(a+b-)phenotypes.
作者
周世航
潘凌子
宋文倩
邵林楠
范亚欣
ZHOU Shihang;PAN Lingzi;SONG Wenqian;SHAO Linnan;FAN Yaxin(Dalian Blood Center,Dalian 116001,China)
出处
《中国输血杂志》
CAS
2023年第10期872-875,共4页
Chinese Journal of Blood Transfusion
基金
辽宁省自然基金指导计划(2019-ZD-0883)。
