摘要
目的:对液态乳中多种阴离子进行快速检测。方法:通过乙腈沉淀液态乳中的大分子蛋白质并提取多种阴离子,经C 18柱净化,利用Dionex IonPac AS19-HC离子色谱柱分离,氢氧化钾梯度洗脱,电导检测,采用离子色谱仪对液态乳中溴酸根、硫氰酸根、亚硝酸根、硝酸根、氯酸根、高氯酸根、磷酸根、碘离子8种常见阴离子进行检测。结果:8种阴离子在线性浓度范围内的相关系数为0.9990~0.9998,检出限为0.065~0.417 mg/kg,回收率为81.4%~102.0%,相对标准偏差为1.1%~6.7%(n=6)。结论:该方法操作简单、准确度和灵敏度高,可用于液态乳中多种阴离子残留量的同时检测。
Objective:A rapid method was established for the simultaneous determination of variety anions in liquid milk.Methods:The macromolecular protein in liquid milk was precipitated by acetonitrile,and a variety of anions were extracted.After purification by C 18 column,the material was separated by Dionex IonPac AS19-HC ion column with gradient elution of potassium hydroxide and conductivity detection.Finally,a method for the determination of bromate,thiocyanate,nitrite,nitrate,chlorate,perchlorate,phosphate and iodine ion in liquid milk was established using ion chromatograph.Results:The results showed that the correlation coefficients(R 2)of eight kinds of anion range from 0.9990 to 0.9998 in the linear range,and the limits of detection were between 0.065 mg/kg and 0.417 mg/kg.The recoveries were in the range 81.8%~102.0%with the relative standard deviations in the range 1.1%~6.7%(n=6).Conclusion:The method has the advantages of simple operation,high accuracy and sensitivity,and can be used for simultaneous determination of various anion residues in liquid milk.
作者
吴滋灵
姚晓庆
沈应涛
黄嘉乐
何敏恒
WU Ziling;YAO Xiaoqing;SHEN Yingtao;HUANG Jiale;HE Minheng(Guangzhou Inspection Testing and Certification Group Co.Ltd.,National Quality Testing Center for Processed Food〔Guangdong〕,Guangzhou,Guangdong 511447,China)
出处
《食品与机械》
CSCD
北大核心
2023年第10期56-61,68,共7页
Food and Machinery
基金
广州检验检测认证集团有限公司科技项目(编号:2019KJ02GZ)。
关键词
液态乳
离子色谱
电导检测
阴离子
liquid milk
ion chromatography
conductivity detection
anionic
