摘要
蚕豆(ViciafabaL.)是我国重要的食用豆作物,在我国南方为秋播作物,主要生产大粒鲜食型蚕豆,对该地区蚕豆资源进行粒型性状关联分析,发掘与其关联的分子标记,有助于鲜食型蚕豆分子标记辅助育种。本研究以90份秋播区蚕豆资源为材料,于2019年和2020年对其粒型性状进行了表型鉴定;采用67对多态性的SSR标记对群体进行基因型鉴定,共扩增出278个等位变异,平均等位基因数目为4.1,聚类分析将该资源分成3大类,其中粒长、粒宽和百粒重3个性状值较大的品种主要分布在A类群。在群体结构分析的基础上,利用TASSEL软件的MLM(Q+K)模型分析了67个SSR标记与粒型性状的相关性,通过关联分析共检测到50个与粒型性状显著相关联的分子标记(P<0.001),其中有29个标记P值小于0.0001。在多年多点的分析中,ICS48和ICS455同时与粒宽和粒重显著关联。根据关联分子标记的稳定性和贡献率,得到3个与粒型相关的优势等位位点:ICS48-H1、ICS51-H1以及ICS455-H3(P<0.0001;r>18%)。本研究将有助于我国秋播区蚕豆的分子标记辅助选择和分子设计育种。
Faba bean(Vicia faba L.)is an important legume crop in China.Southern China is the autumn planting area of faba bean,where mainly produces large-grained fresh faba bean.The correlation analysis of the seed size-related traits of the faba bean resources from this area was carried out to explore the associated molecular markers applicable for molecular marker-assisted breeding.In this study,phenotype of seed sizerelated traits in 90 faba bean resources were collected in 2019 and 2020.Sixty-seven pairs of polymorphic SSR markers were used for genotyping,producing a total of 278 alleles with the average alleles of 4.1.The resources were divided into 3 groups by cluster analysis,in which accessions with higher values of seed length,seed width and 100-seed weight are mainly found in the A group.In addition to the population structure analysis,the MLM models(Q+K)of TASSEL software were used to conduct associate studies of seed size-related traits and 67 SSR markers.A total of 50 significantly associated markers(P<0.001)were detected by mixed linear model(MLM)of TASSEL,of which 29 markers had a P-value less than 0.0001.In multiple environments analysis,ICS48 and ICS455 were significantly associated with both grain width and grain weight.According to the association stability and contribution rate,three dominant alleles(ICS48-H1,ICS51-H1 and ICS455-H3)related to seed size were obtained(P<0.0001;r>18%).This study will contribute to the molecular marker-assisted selection and molecular design breeding of faba bean in autumn planting areas in China.
作者
刘金洋
周琰琰
林云
刘萌萌
薛晨晨
陈景斌
闫强
吴然然
陈新
袁星星
LIU Jinyang;ZHOU Yanyan;LIN Yun;LIU Mengmeng;XUE Chenchen;CHEN Jingbin;YAN Qiang;WU Ranran;CHEN Xin;YUAN Xingxing(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory of Efficient Horticultural Crop Genetic Improvement,Nanjing 210014)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2023年第6期1602-1614,共13页
Journal of Plant Genetic Resources
基金
国家食用豆产业技术体系(CARS-08-G15)
江苏省种业振兴“揭榜挂帅”项目(JBGS[2021]056)。
关键词
蚕豆
遗传多样性
粒型
SSR关联分析
faba bean
genetic diversity
seed shape
SSR association analysis
