摘要
目的 本研究拟对比两种不同高脂饮食方式诱导的高脂血症大鼠肠道菌群变化与短链脂肪酸代谢特征,以宿主-肠道菌群-代谢角度探讨高脂血症可能的微观机制。方法 SPF级SD大鼠分为:正常饮食组(CG组):饲喂大鼠维持饲料;高脂饮食组(HFD1组):每天足量饲喂高脂饲料;限饲高脂饮食组(HFD2组):每天限量饲喂高脂饲料80 g,不限量饲喂维持饲料。8周后检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平;苏木精-伊红(HE)染色观察大鼠肝组织和肾周脂肪病理学变化;取结肠内容物进行16S rDNA高通量测序,观察肠道菌群结构与功能的变化,并检测结肠内容物中短链脂肪酸的含量。结果 与CG组相比,HFD1组和HFD2组大鼠摄食量下降,体重升高;血清中TC、TG、LDL-C均显著升高;肝组织发生明显脂肪变性,肾周脂肪出现炎性病变;高脂干预后大鼠肠道菌群相对丰度显著变化,其中乳杆菌属相对丰度明显降低,菌群结构和功能变化明显,总短链脂肪酸、乙酸、丁酸、异丁酸下降显著。结论 两种高脂饮食方式均能引起大鼠高脂血症,且发病机制基本一致,均与脂质代谢以及肠道菌群紊乱有关。每日限制一定的高脂饲料饲喂量不仅能够降低高脂饲料对大鼠食欲的影响,还能提高大鼠高脂血症模型制备的稳定性,是一种值得推广应用的大鼠高脂模型制备方法。
Objective To compare changes in intestinal flora and the characteristics of short-chain fatty acid metabolism in hyperlipidemic rats induced by two different high fat diets,and to explore the possible mechanism of hyperlipidemia from the viewpoint of host-intestinal flora metabolism.Methods SPF SD rats were divided into a normal diet group(CG)and two high fat diet groups(HFD1 and HFD2).Rats were fed an 80 g high fat diet and unlimited maintenance diet.After 8 weeks,serum levels of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),and high density lipoprotein cholesterol(HDL-C)were measured.Pathological changes in liver tissue and perirenal fat were observed by hematoxylin-eosin(HE)staining.Colon contents were collected for 16S rDNA high-throughput sequencing to assess changes in the structure and function of intestinal flora and the intestinal contents of short-chain fatty acids.Results Compared with the CG group,food intake was decreased and body weight was increased,serum TC,TG,and LDL-C were increased significantly,liver tissue showed obvious steatosis and perirenal fat showed inflammatory lesions in HFD1 and HFD2 groups.After high fat intervention,the relative abundance of intestinal microflora in rats was changed significantly and sex differences were observed,in which the relative abundance of Lactobacillus was decreased significantly,and the structure and function of Lactobacillus were decreased significantly,including total short-chain fatty acids,acetic acid,butyric acid,and isobutyric acid.Conclusions The two kinds of high-fat diet caused hyperlipidemia in rats.The pathogenesis was essentially the same and related to lipid metabolism and intestinal flora disorder.Daily restriction of a certain amount of high fat diet reduced the effect of high fat on appetite and improved stability of rat hyperlipidemia model establishment.
作者
杨宗统
孙铁锋
李晓晶
徐东川
袁敏
靳光乾
王雯慧
YANG Zongtong;SUN Tiefeng;LI Xiaojing;XU Dongchuan;YUAN Min;JIN Guangqian;WANG Wenhui(College of Veterinary Medicine,Gansu Agriculture University,Lanzhou 730070;China.2.Shandong Academic of Chinese Medicine,Jinan 250014)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2023年第10期1314-1323,共10页
Acta Laboratorium Animalis Scientia Sinica
基金
山东省技术创新引导计划(鲁渝科技协作)(2020LYXZ011)。
关键词
高脂血症
肠道菌群
短链脂肪酸
发病机制
hyperlipidemia
gut microbiota
short-chain fatty acids
pathogenesis
