摘要
目的:建立MFC335多功能柱净化-超高效液相色谱-串联质谱检测米酵菌酸的方法。方法:样品提取后,经MFC335多功能柱净化。以0.1%甲酸水和乙腈为流动相梯度洗脱,用ThermocHypersil Gold C_(18)色谱柱分离,ESI–进行多反应监测,外标法定量。结果:米酵菌酸的线性范围为5.0~200μg/L、相关系数(r)>0.999、检出限2μg/kg、定量限6μg/kg。3个加标水平下(100、400、900μg/kg)的回收率为87.5%~112%,RSD为5.21%~9.93%。结论:该方法快速准确,可用于米酵菌酸的定性、定量检测。
A method for the determination of bongkrekic acid by ultra performance liquid chromatographytandem mass spectrometry(UPLC-MS)with MFC335 multifunctional column purification was established.After extraction,the sample was purified by MFC335 multifunctional column,eluted with 0.1%formic acid water and acetonitrile as mobile phase gradient,separated by ThermocHypersil Gold C_(18) chromatographic column,ESI–was used for multiple reaction monitoring(MRM),and external standard method was used for quantification.Good linearity was obtained for the bongkrekic acid within the concentration range of 5.00–200μg/L,with the correlation coefficients(r)higher than 0.999.The limit of detection(LOD)and the limit of quantification(LOQ)of the bongkrekic acid was 2μg/kg and 6μg/kg,respectively.The average recovery rate at three concentrations of 100,400,900μg/kg was in the range of 87.5%–112%with relative standard deviations of 5.21%–9.93%.The results showed that this method could be used to determine the quality and quantity of bongkrekic acid rapidly and accurately.
作者
孟春杨
吴玉田
王颖怡
彭蕾
刘文政
周贻兵
MENG Chunyang;WU Yutian;WANG Yingyi;PENG Lei;LIU Wenzheng;ZHOU Yibing(Guizhou Provincial Center for Disease Control and Prevention,Guiyang 550004,China)
出处
《食品科技》
CAS
北大核心
2023年第8期309-313,共5页
Food Science and Technology
基金
贵州省卫生健康委科学技术基金项目(gzwkj2022-268)
贵州省科学(青年)技术基金项目(2021-E1-2青)。
