摘要
目的:探讨黄芪甲苷(AS-IV)对牙周炎(PD)大鼠正畸牙齿移动(OTM)过程中炎症反应和牙周组织重塑的影响及分子机制。方法:18只Wistar雄性大鼠纳入正常对照(NC)组,72只用于结扎法诱导建立PD模型,建模后分为PD组、PD+OTM组、PD+OTM+AS-IV组、PD+OTM+AS-IV+LPS组,每组18只。持续喂养4周,检测牙周组织TNF-α、IL-6、IL-1β水平,MMP-2、MMP-9、RANKL和OPG mRNA表达,分析牙周组织TLR4/MyD88/NF-κB通路相关蛋白表达。结果:与NC组相比,PD组大鼠CEJ-ABC距离、牙周组织损伤评分、多核破骨细胞数量、NF-κB p65阳性表达、TNF-α、IL-1β、IL-6水平、MMP-2、MMP-9和RANKL mRNA水平以及RANKL/OPG比值、TLR4、MyD88和核NF-κB p65蛋白水平增加,骨组织骨体积百分比(BV/TV)、骨小梁数(Tb.N)和骨小梁厚度(Tb.Th)降低(均P<0.05);PD+OTM进一步加剧了PD大鼠上述指标的变化,加重了牙周炎症反应和牙槽骨丢失。PD+OTM+AS-IV组上述指标变化向NC组接近,PD+OTM+AS-IV组上述指标向PD+OTM组接近。结论:AS-IV可抑制炎症反应,减少PD大鼠OTM期间的牙槽骨丢失,改善牙周组织重塑,其作用机制可能与抑制TLR4/MyD88/NF-κB信号通路有关。
Objective:To investigate the impacts and molecular mechanism of astragaloside IV(AS-IV)on periodontal tissue remo-deling during orthodontic tooth movement(OTM)in rats with periodontitis(PD).Methods:18 Wistar male rats without treatment were included in normal control group(NC group).72 rats were induced for the establishment of PD models by ligation.After modeling,the rats were divided into PD group,PD+OTM group,PD+OTM+AS-IV group,and PD+OTM+AS-IV+LPS group(n=18).4 weeks after modelling,the levels of TNF-α,IL-6 and IL-1β,the mRNA expression MMP-2,MMP-9,RANKL-κB and OPG in periodontal tissues were examined,the expression of TLR4/MyD88/NF-κB pathway-related proteins in periodontal tissues was analyzed.Results:Compared with the NC group,the CEJ-ABC distance,periodontal tissue damage score,the number of multinucleated osteoclasts,the positive expression of NF-κB p65,the levels of TNF-α,IL-1βand IL-6,the mRNA levels of MMP-2.MMP-9 and RANKL,the RANKL/OPG ratio,the protein levels of TLR4,MyD88 and nuclear NF-κB p65 of the rats in PD group were significantly increased,the bone volume percentage(BV/TV),trabecular bone number(Tb.N)and trabecular bone thickness(Tb.Th)were significantly reduced(all P<0.05);PD+OTM aggravated the changes of the above indexes in PD rats,aggravated periodontal inflammation and alveolar bone loss.In PD+OTM-AS-IV group,the above indexes were closer to those in NC group.In PD+OTM+AS+IV+LPS group,the above indexes were closer to those of PD+OTM group.Conclusion:AS-IV can inhibit inflammatory response,reduce alveolar bone loss during OTM in PD rats,and improve periodontal tissue remodeling.Its mechanism may be related to the inhibition of TLR4/MyD88/NF-κB signaling pathway.
作者
王丹
刘雨笛
邵丽萍
马稔秋
WANG Dan;LIU Yudi;SHAO Liping;MA Renqiu(Department of Stomatology,Affiliated Hospital of Chifeng University,Inner Mongolia,024000,China)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2023年第6期722-729,共8页
Journal of Practical Stomatology
