摘要
目的 建立玉叶金花总皂苷指纹图谱,并进行其肝保护作用的谱效关系研究。方法 取不同产地的玉叶金花药材,以75%乙醇为溶剂,制备10批玉叶金花总皂苷。采用高效液相色谱法测定,利用《中药色谱指纹图谱相似度评价系统(2012版)》绘制10批玉叶金花总皂苷的指纹图谱,并进行相似度评价、共有峰指认。采用同一高效液相色谱法,测定其中5种三萜皂苷类成分(mussaendoside H、mussaendoside U、mussaglaoside C、mussaendoside G、mussaendoside O)的含量。以四氯化碳致急性肝损伤小鼠模型考察玉叶金花总皂苷的肝保护作用,并通过灰色关联度分析法进行谱效关系研究。结果 10批玉叶金花总皂苷共有11个共有峰,指认了其中5个,分别为mussaendoside H(3号峰)、mussaendoside U(7号峰)、mussaglaoside C(8号峰)、mussaendoside G(9号峰)、mussaendoside O(11号峰);10批样品的相似度为0.940~0.991。mussaendoside H、mussaendoside U、mussaglaoside C、mussaendoside G、mussaendoside O的平均含量分别为0.01~0.05、0.10~0.21、0.10~0.18、0.03~0.08、0.20~0.40 mg/g。10批玉叶金花总皂苷可普遍降低模型小鼠血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)含量和肝组织中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、IL-1β含量(P<0.05或P<0.01)。各共有峰峰面积与ALT、AST、TNF-α、IL-6、IL-1β含量的关联度分别为0.602~0.757、0.585~0.833、0.593~0.795、0.618~0.820、0.607~0.804,关联度均较大的依次为11、9、8号峰。结论 10批玉叶金花总皂苷成分相似,mussaendoside H、mussaendoside U、mussaglaoside C、mussaendoside G、mussaendoside O的平均含量不一;10批样品均有一定的肝保护作用,mussaendoside O、mussaendoside G、mussaglaoside C可能是其主要药效成分。
OBJECTIVE To establish the fingerprint of total saponins from Mussaenda pubescens,and to study the spectrum-effect relationship of its hepatoprotective activity.METHODS Ten batches of total saponins from M.pubescens from different origins were prepared using 75%ethanol as solvent.High-performance liquid chromatography(HPLC)and the Similarity Evaluation System for Traditional Chinese Medicine Chromatographic Fingerprints(2012 edition)were used to draw the fingerprints of 10 batches of total saponins from M.pubescens.The similarity evaluation and identification of common peaks were conducted.The same HPLC method was adopted to determine the contents of five triterpenoid saponins(mussaendoside H,mussaendoside U,mussaglaoside C,mussaendoside G and mussaendoside O).The hepatoprotective effect of total saponins from M.pubescens was investigated by establishing carbon tetrachloride-induced acute liver injury model mice,and the spectrum-effect relationship was studied by using grey correlation analysis.RESULTS There were 11 common peaks in 10 batches of total saponins from M.pubescens,5 of which were identified,i.e.mussaendoside H(peak 3),mussaendoside U(peak 7),mussaglaoside C(peak 8),mussaendoside G(peak 9)and mussaendoside O(peak 11);the similarities of 10 batches of samples ranged 0.940-0.991.Average contents of mussaendoside H,mussaendoside U,mussaglaoside C,mussaendoside G,mussaendoside O were 0.01-0.05,0.10-0.21,0.10-0.18,0.03-0.08,0.20-0.40 mg/g,respectively.Ten batches of total saponins from M.pubescens could generally reduce the contents of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum,and tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and IL-1βin liver tissue of model mice(P<0.05 or P<0.01).The correlation between the common peak areas and the contents of ALT,AST,TNF-α,IL-6 and IL-1βwere 0.602-0.757,0.585-0.833,0.593-0.795,0.618-0.820,0.607-0.804,respectively;the peaks with high correlation were peaks 11,9 and 8 in order.CONCLUSIONS Ten batches of total saponins from M.pubesc
作者
张颖
罗金荣
张赟赟
陈锋
何飞
李嘉
ZHANG Ying;LUO Jinrong;ZHANG Yunyun;CHEN Feng;HE Fei;LI Jia(Guangxi Institute of Chinese Medicine&Pharmaceutical Science,Nanning 530022,China;Guangxi Key Laboratory of the Quality Standard of Traditional Chinese Medicine,Nanning 530022,China)
出处
《中国药房》
CAS
北大核心
2023年第22期2715-2720,共6页
China Pharmacy
基金
广西自然科学基金项目(No.2020GXNSFAA297180)。
关键词
玉叶金花
总皂苷
指纹图谱
含量测定
肝保护作用
谱效关系
Mussaenda pubescens
total saponins
fingerprint
content determination
hepatoprotective activity
spectrum-effect relationship
