摘要
目的 对核通道蛋白37 kDa(nucleoporin 37 kDa, NUP37)进行泛癌分析,观察NUP37对胰腺癌(pancreatic adenocarcinoma, PAAD)细胞SW1990增殖、侵袭及迁移的影响。探讨NUP37在PAAD中的表达差异、临床意义及预后价值,并预测NUP37在PAAD中可能的作用机制。方法 从癌症基因组图谱(The Cancer Genome Atlas, TCGA)数据库下载与整理NUP37在33种肿瘤及11 057例样本的mRNA表达水平及生存预后相关数据,统计分析NUP37在泛癌中的表达及预后情况。分别采用5-乙炔基-2′脱氧尿嘧啶核苷(5-ethynyl-2′-deoxyuridine, EdU)免疫荧光实验、Transwell实验、划痕实验检测过表达NUP37和沉默NUP37对SW1990细胞增殖、侵袭、迁移的影响。利用基因表达水平值的交互式分析平台(Gene Expression Profiling Interactive Analysis, GEPIA)数据库分析NUP37在PAAD中的表达及预后。从TCGA数据库获取PAAD患者NUP37表达水平、临床病理特征及生存时间等数据,统计分析NUP37表达水平与PAAD患者临床病理特征及预后的关系。通过基因组富集分析(Gene Set Enrichment Analysis, GSEA)预测NUP37在PAAD中可能调控的信号通路。结果 泛癌分析结果显示,NUP37在膀胱尿路上皮癌(bladder urothelial carcinoma, BUC)、乳腺浸润癌(breast invasive carcinoma, BRCA)、胆管癌(cholangiocarcinoma, CHOL)等17种肿瘤中高表达(P<0.05),且NUP37高表达预示着PAAD、肺腺癌(lung adenocarcinoma, LUAD)、肝细胞性肝癌(liver hepatocellular carcinoma, LIHC)等7种肿瘤患者的生存状态更差(P<0.05)。GEPIA数据库分析结果表明,NUP37在PAAD组织中的mRNA表达水平显著高于正常胰腺组织(P<0.05),且NUP37高表达组PAAD患者的总生存期显著低于低表达组患者(P<0.05),预后更差。EdU免疫荧光实验检测结果显示,过表达NUP37促进PAAD SW1990细胞增殖(P<0.01),沉默NUP37则抑制PAAD SW1990细胞增殖(P<0.01)。Transwell实验检测结果显示,过表达NUP37促进PAAD SW1990细胞侵袭(P<0.001),沉默NUP37则抑
Objective To investigate pan-cancer analysis of nucleoporin 37 kDa(NUP37), and to observe the effects of NUP37 on the proliferation, invasion and migration of pancreatic adenocarcinoma(PAAD) cell SW1990. Moreover, to investigate the expression difference, clinical significance and prognostic value of NUP37 in PAAD, and to predict the possible mechanism of NUP37 in PAAD. Methods mRNA expression levels and survival prognosis related data of NUP37 in 33 types of tumors and 11 057 samples were downloaded and organized from The Cancer Genome Atlas(TCGA) database, and the expression and prognosis of NUP37 in pan cancer were statistically analyzed. The effects of over expresson NUP37 and silencing NUP37 on the proliferation, invasion and migration of SW1990 cells were detected by 5-ethynyl-2′-deoxyuridine(EdU) immunofluorescence assay, Transwell assay and scratch assay. The expression and prognosis of NUP37 in PAAD were analyzed by Gene Expression Profiling Interactive Analysis(GEPIA) database. NUP37 expression level, clinicopathological characteristics and survival time of PAAD patients were obtained from TCGA database, and the relationship between NUP37 expression level and clinicopathological characteristics, prognosis of PAAD patients were statistically analyzed. The possible regulatory signaling pathway of NUP37 in PAAD was predicted by Gene Set Enrichment Analysis(GSEA). Results The pan cancer analysis results showed that NUP37 was highly expressed in 17 kinds of tumors, including bladder urothelium carcinoma(BUC), breast invasive carcinoma(BRCA) cholangiocarcinoma(CHOL), etc.(P<0.05), and the high expression of NUP37 indicated that the survival status of patients with 7 kinds of tumors was worse, including PAAD, lung adenocarcinoma(LUAD), liver hepatocellular carcinoma(LIHC), etc.(P<0.05). GEPIA database analysis results showed that the mRNA expression level of NUP37 PAAD tissue was significantly higher than that in normal pancreatic tissue(P<0.05), and the overall survival period of patients with PAAD in the N
作者
吴海斌
匡鹏
马丹丹
龚齐
董庆泰
傅涛
WU Haibing;KUANG Peng;MA Dandan;GONG Qi;DONG Qingtai;FU Tao(Department of General Surgery,General Hospital of Central Theater Command,Wuhan Hubei 430070,China)
出处
《联勤军事医学》
CAS
2023年第9期737-742,747,共7页
Military Medicine of Joint Logistics
基金
湖北省卫生健康委科研项目(WJ2023F038)。
关键词
核通道蛋白37
kDa
泛癌
胰腺癌
表达
细胞功能
预后
Nucleoporin 37 kDa
Pan cancer
Pancreatic cancer
Expression
Cell function
Prognosis
