摘要
目的:探究地奥心血康(DXXK)基于肠道菌群改善阿霉素致心脏毒性的作用。方法:36只SD大鼠随机分为对照组、模型组、阳性组、心血康低、中、高剂量组,每组6只。模型组、阳性组和心血康组腹腔注射阿霉素(2.5 mg·kg^(-1),每周1次,共6周),第1次造模同时,按25 mg·kg^(-1)腹腔注射右丙亚胺,每周1次,共6周;分别按50、100、200 mg·kg^(-1)灌胃给药心血康,对照组和模型组灌胃等量蒸馏水,每天1次,连续6周。末次给药后,进行心电图检测;HE染色观察心肌损伤程度;ELISA检测心脏组织CK-MB、LDH、BNP、c Tn T、IL-1β、IL-6、TNF-α水平;取对照组、模型组、心血康高剂量组的新鲜粪便,采用高通量16S r RNA测序分析。结果:心血康可以显著恢复P波幅度、T波幅度、R波幅度、ST波幅度、S波幅度、Q波幅度,改善心肌纤维化和炎性浸润,降低心脏CK-MB、LDH、BNP、c Tn T、IL-1β、IL-6、TNF-α水平,DXXK最有效的剂量为200 mg·kg^(-1)。还能够调整心肌损伤大鼠的菌群丰度及多样性,并恢复异常偏移的菌群结构,使其贴近正常水平。与对照组比较,模型组的厚壁菌门丰度升高,拟杆菌门丰度降低,F/B值增大。给药后心血康组厚壁菌门丰度降低,拟杆菌门的丰度升高,F/B值减小。属水平上,地奥心血康可恢复因心衰导致的菌属结构紊乱,同时提高肠道有益菌丰度,保护肠粘膜。LEf Se结果表明,阿霉素的干预促进了异杆菌的增殖,损害肠道健康,而心血康可以通过上调艾克曼菌的活性改善肠道内环境。结论:地奥心血康可以调控阿霉素致心肌损伤大鼠的肠道微生物环境,可能通过恢复菌群结构,提高益生菌活性并抑制致病菌生长等途径,改善因心肌损伤导致的大鼠肠道菌群紊乱。
Objective:To investigate the effect of Di'aoxinxuekang (DXXK) on intestinal bacteria in rats with myocardial injury induced by DOX.Method:36 SD rats were randomly divided into control group,model group,positive group,low-dose,mediumdose and high-dose groups with 6 rats in each group.Model group,positive group and drug administration group were intraperitoneally injected with DOX (2.5 mg·kg^(-1),once a week,for 6 weeks).At the same time of the first modeling,DXXK was intragastric administered at 50,100 and 200 mg·kg^(-1),respectively.The control group and model group were intragastric administered with the same amount of distilled water,once a day,for 6 weeks.After the last administration,control group and model group were selected,Fresh feces of DXXK high-dose group were analyzed by high-throughput 16S r RNA sequencing.Result:DXXK could significantly restore myocardial cell membrane potential,improve myocardial fibrosis and inflammatory infiltration,reduce cardiac inflammation and myocardial enzyme levels,and adjust the abundance and diversity of bacterial community in rats with myocardial injury,and restore the abnormal deviated bacterial community structure,making it close to the normal level.Compared with the control group,Firmicutes abundance was increased in model group,Bacteroidetes abundance was decreased,and F/B value was increased.After administration,the abundance of Firmicutes was decreased,the abundance of Bacteroidetes was increased,and the F/B value was decreased in DXXK group.At the same time,DXXK can restore the intestinal bacterial structure disorder caused by heart failure,improve the abundance of intestinal beneficial bacteria and protect intestinal mucosa.LEf Se results showed that the intervention of DOX promoted the proliferation of heterobacilli and damaged intestinal health,while DXXK could improve the intestinal environment by up-regulating the activity of Ekman bacteria.Conclusion:DXXK can regulate the intestinal microbial environment of rats with myocardium injury caused by doxorubici
作者
袁楚桥
王涛
邹文俊
YUAN Chu-qiao;WANG Tao;ZOU Wen-jun(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan)
出处
《中药与临床》
2023年第5期60-65,共6页
Pharmacy and Clinics of Chinese Materia Medica
基金
成都中医药大学杏林学者学科人才科研提升计划(CXTD2018005)。
关键词
地奥心血康
阿霉素
心肌损伤
肠道菌群
Di'aoxinxuekang
doxorubicin
myocardial injury
intestinal flora
