摘要
甘油二酯激酶(DGK)基因介导的磷脂酸(PA)合成是动植物细胞中重要的信号转导过程,在逆境胁迫应答中发挥重要的调控作用。前期通过对苹果DGK基因家族成员全基因组分析,鉴定到一个MpDGK7基因,其基因表达受干旱、高盐以及外源ABA处理调控。为进一步探究该基因在响应逆境胁迫过程中的生物学功能,本研究将MpDGK7基因异源转化拟南芥,对MpDGK7过表达拟南芥株系抗旱性进行鉴定,叶片气孔特征进行观察,并测定胁迫处理后过氧化氢(H_(2)O_(2))、丙二醛(MDA)含量和抗氧化系统相关酶活性。结果发现,MpDGK7基因过量表达能够提高拟南芥的抗旱性,促进胁迫条件下气孔迅速关闭,降低植株体内H_(2)O_(2)的积累并提高抗氧化酶(如CAT、POD和APX)的活性。综上,MpDGK7基因通过调节气孔运动、改进抗氧化系统参与了对干旱胁迫的信号转导。本研究结果为进一步探索DGK基因生物学功能及其作用机制奠定了基础。
Phosphatidic acid(PA)synthesis mediated by diacylglycerol kinase(DGK)gene is an important signal transduction process in animal and plant cells and plays an important regulatory role in stress response.MpDGK7 gene was identified by genome-wide analysis of apple DGK gene family,and its expression was regulated by drought,high salt and exogenous ABA treatment.In order to further explore the biological function of this gene in response to stress,the MpDGK7 gene was heterogenetically transformed into Arabidopsis.Drought resistance of Arabidopsis strains overexpressed MpDGK7 was identified and stomatal characteristics of leaves were observed.After stress treatment,the contents of hydrogen peroxide(H_(2)O_(2)),malondialdehyde(MDA)and the activities of enzymes related to antioxidant system were determined.It was found that overexpression of MpDGK7 gene could improve the drought resistance of Arabidopsis and promote the rapid closure of stomata under stress,reduce the accumulation of H_(2)O_(2)in plants and increase the activity of antioxidant enzymes(such as CAT,POD and APX).In conclusion,MpDGK7 gene is involved in drought stress signal transduction by regulating stomatal movement and improving antioxidant system.The results of this study laid a foundation for further exploration of the biological function and mechanism of DGK gene.
作者
谭延肖
韩梅梅
张超
常培培
徐文胜
TAN Yanxiao;HAN Meimei;ZHANG Chao;CHANG Peipei;XU Wensheng(Dezhou Academy of Agricultural Sciences,Dezhou,Shandong 253015)
出处
《核农学报》
CAS
CSCD
北大核心
2023年第9期1729-1734,共6页
Journal of Nuclear Agricultural Sciences
基金
山东省自然科学基金资助项目(ZR2021QC035)。
