摘要
目的构建掺杂铕(Eu)的中空介孔二氧化硅(SiO2)纳米粒子载药平台,并观察其特性。方法采用水热法制备掺杂Eu的SiO2空心微球,在其表面修饰靶向物质透明质酸(HA),以浸润法将紫杉醇(PTX)负载于介孔氧化硅纳米颗粒(MSN)中,得到HA-Eu-hMSNs-PTX,观察其形态、荧光特性等性状,以及载药量、生物安全性及细胞毒性。结果所获HA-Eu-hMSNs直径(61.33±8.94)nm;以PTX修饰后成功制备HA-Eu-hMSNs-PTX,其PTX负载量为52.33µg/mg。加入Eu-hMSNs和HA-Eu-hMSNs后,SW1990细胞的细胞核均被DAPI染为蓝色并可见红色荧光信号,但加入Eu-hMSNs后该信号较弱。加入HA-Eu-hMSNs 24 h后,最高浓度(200μg/ml)组SW1990细胞存活率仍在90%以上。PTX、Eu-hMSNs-PTX和HA-Eu-hMSNs-PTX均对SW1990细胞具有浓度依赖性细胞毒性;相同PTX浓度下,HA-Eu-hMSNs-PTX的细胞毒性高于游离PTX和Eu-hMSNs-PTX。结论所制备HA-Eu-hMSNs-PTX粒径均匀、生物安全性佳,靶向能力和肿瘤细胞杀伤能力良好。
Objective To construct a europium(Eu)doped hollow mesoporous silica(SiO2)nanoparticles drug delivery platform,and to observe its characteristics.Methods Eu doped SiO2 hollow microspheres were prepared using hydrothermal method.The targeting substance hyaluronic acid(HA)was modified on the surface of the above nanoparticles,and paclitaxel(PTX)was loaded into mesoporous silica nanoparticles(MSN)using infiltration method to prepare HA-Eu-hMSNs-PTX.The morphology,fluorescence characteristics,drug loading,biosafety and cytotoxicity of HA-Eu-hMSNs-PTX were observed.Results The diameter of HA-Eu-hMSNs was(61.33±8.94)nm.After being modified with PTX,HA-Eu-hMSNs-PTX was successfully prepared,with PTX loading capacity of 52.33µg/mg.The nuclei of SW1990 cells were stained as blue by Eu-hMSNs and HA-Eu-hMSNs,both with red fluorescence signals,while the signals of cells with Eu-hMSNs was weaker than with HA-Eu-hMSNs.The survival rate of SW1990 cells remained>90%24 h after administration of the highest concentration(200μg/ml)of HA-Eu-hMSNs.PTX,Eu-hMSNs-PTX and HA-Eu-hMSNs-PTX all were found with concentration dependent cytotoxicity to SW1990 cells.At the same PTX concentration,cytotoxicity of HA-Eu-hMSNs-PTX was higher than that of PTX and Eu-hMSNs-PTX.Conclusion The successfully prepared HA-Eu-hMSNs-PTX had uniform particle size,good biosafety,excellent targeting ability and tumor cell killing capability.
作者
韩慧敏
杨子熙
王嘉遥
贾媛媛
李忠涛
郝利国
HAN Huimin;YANG Zixi;WANG Jiayao;JIA Yuanyuan;LI Zhongtao;HAO Liguo(College of Medical Technology,Qiqihar Medical University,Qiqihar 161006,China;College of Basic Medical Sciences,Qiqihar Medical University,Qiqihar 161006,China)
出处
《中国介入影像与治疗学》
北大核心
2023年第11期689-693,共5页
Chinese Journal of Interventional Imaging and Therapy
基金
2022年度黑龙江省大学生创新创业训练计划项目(202211230020)。
关键词
二氧化硅
纳米微粒
紫杉醇
药物载体
铕
抗肿瘤药
silicon dioxide
nanoparticles
paclitaxel
drug carriers
europium
antineoplastic agents
