摘要
目的 研究吡啶硫酮铜(Cu PT)对凡纳滨对虾Litopenaeus vannamei肝胰腺能量利用、脂质过氧化相关指标和抗氧化、凋亡相关基因表达量的影响。方法 设置空白对照组、DMSO溶剂对照组(0.128 m L·L~(-1))和2个Cu PT处理组(64μg·L~(-1)和128μg·L~(-1)),每组3个平行,在200 L水体中暴露48 h,分别于胁迫0 h、3 h、12 h、24 h和48 h取肝胰腺进行测定。结果 随着Cu PT暴露浓度的增加及胁迫时间的延长,凡纳滨对虾肝胰腺中的糖原、乳酸含量显著下降(P<0.05),丙二醛含量显著升高(P<0.05);锰超氧化物歧化酶基因、谷胱甘肽过氧化物酶基因、过氧化氢酶基因和凋亡相关基因半胱氨酸蛋白酶3基因和肿瘤抑制基因的表达水平也发生了显著变化(P<0.05)。结论 CuPT可能影响凡纳滨对虾正常能量供应,并使机体出现脂质过氧化,还会影响机体内抗氧化应激以及凋亡相关基因的表达水平。
Objective The effects of copper pyrithione(CuPT)on hepatopancreas energy utilization,lipid peroxidation-related indexes,and the expression of antioxidant and apoptosis-related genes in Litopenaeus vannamei were studied.Methods L.vannamei grew in 200 L water containing DMSO(0.128 mL·L-1),64μg·L-1 or 128μg·L-1 of CuPT for 48 h.The hepatopancreaswere obtained at 0 h,3 h,12 h,24 h,and 48 h after exposure,respectively.Results With the increase of concentration of CuPT and the exposure time,the contents of glycogen and lactic acid decreased significantly(P<0.05)compared to the control group,while the content of malondialdehyde increased significantly(P<0.05).At the same time,the expression levels of antioxidant-encoding genes MnSOD,GPx and CAT,and apoptosis-related genes cas-pase-3 and p53 changed significantly(P<0.05).Conclusion CuPT may disrupt the normal energy supply and cause lipid peroxidation of L.vannamei,and also affects the expression levels of antioxidant stress and apoptosis-related genes.
作者
李舒泓
陈天赐
赵永鹏
李呈浩
梁植
郭慧
LI Shuhong;CHEN Tianci;ZHAO Yongpeng;LI Chenghao;LIANG Zhi;GUO Hui(Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang,College of Fisheries,Guangdong Ocean University,Zhanjiang,Guangdong Province 524025,China)
出处
《四川动物》
北大核心
2023年第6期609-617,共9页
Sichuan Journal of Zoology
关键词
吡啶硫酮铜
凡纳滨对虾
肝胰腺
能量利用
脂质过氧化
基因表达
copper pyrithione(CuPT)
Litopenaeus vannamei
hepatopancreas
energy utilization
lipid peroxidation
gene expression
