摘要
目的:利用高效液相色谱法(HPLC)建立不同产地川产枳壳特征图谱,结合熵权法和灰色关联度对川产枳壳药材质量差异性进行分析与评价。方法:采用Agilent Eclipse Plus C18色谱柱(4.6 mm×250 mm,5μm),以甲醇(A)-0.1%磷酸水溶液(B)为流动相梯度洗脱(0~12 min,25%~33%A;12~21 min,33%~41%A;21~30 min,41%~42%A;30~40 min,42%~59%A;40~53min,59%~72%A;53~60min,72%A;60~65min,72%~100%A;65~70min,100%A;70~71min,100%~25%A;71~80min,25%A),流速1.0 mL·min^(-1),进样量10μL,检测波长330 nm。对50批不同产地(四川、重庆、江西、湖南)枳壳药材进行检测,利用中药色谱指纹图谱相似度评价软件(2012版)生成特征图谱并指认色谱峰,进行相似度评价。采用IBM SPSS 19.0、SIMCA14.1等软件对50批枳壳药材结果进行多元统计分析,利用熵权法和灰色关联度计算川产枳壳质量综合得分。结果:建立了川产枳壳的特征图谱,通过对照品比对指认了14个共有峰,分别为圣草次苷、新北美圣草苷、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、水合橙皮内酯、枸橘苷、橙皮内酯、马尔敏、川陈皮素、3,5,6,7,8,3′,4′-七甲氧基黄酮、橘红素、橙皮油素,川产酸橙枳壳样品特征图谱与对照特征图谱的相似度均>0.980。通过化学模式识别可将样品按主产地分为四类,聚类分析、主成分分析、正交偏最小二乘法-判别分析的结果均能有效辨析不同主产地枳壳样品。熵权法结合灰色关联度综合评价结果显示,不同产地的川产枳壳质量差异较大,川产枳壳的质量排序依次为巴中>泸州>重庆>内江。结论:本研究建立了川产枳壳特征图谱,结合化学计量学与灰色关联度等分析方法,可有效区分不同产地川产枳壳的质量,为川产枳壳质量的综合评价与控制提供参考。
Objective:High performance liquid chromatography(HPLC)was used to establish the specific chromatograms of Aurantii Fructus from different origins,and the quality variability of Aurantii Fructus from Sichuan was analyzed and evaluated by combining entropy weighting method and grey correlation method.Method:HPLC was performed on an Agilent Eclipse Plus C18 column(4.6 mm×250 mm,5μm)with a gradient elution of methanol(A)-0.1%phosphoric acid aqueous solution as the mobile phase(0-12 min,25%-33%A;12-21 min,33%-41%A;21-30 min,41%-42%A;30-40 min,42%-59%A;40-53 min,59%-72%A;53-60 min,72%A;60-65 min,72%-100%A;65-70 min,100%A;70~71 min,100%-25%A;71-80 min,25%A)at a flow rate of 1.0 mL·min^(-1),the injection volume was 10μL and the detection wavelength was 330 nm.Fifty batches of Aurantii Fructus samples from different origins(Sichuan,Chongqing,Jiangxi and Hunan)were tested,and the similarity evaluation software is used to generate characteristic profiles and compare them with control profile for peak identification,and then to evaluate the similarity of the samples.IBM SPSS 19.0 and SIMCA 14.1 were used to perform multivariate statistical analysis on the results of the samples,and then the entropy weighting method and grey correlation were used to calculate the overall quality score of samples from Sichuan.Result:HPLC specific chromatogram of Aurantii Fructus was established,and 14 common peaks were identified as eriocitrin,neoeriocitrin,narirutin,naringin,hesperidin,neohesperidin,meranzin hydrate,poncirin,meranzin,marmin,nobiletin,3,3′,4′,5,6,7,8-heptamethoxyflavone,tangeretin and auraptene.And the similarities between the samples from Sichuan and the control chromatogram were all above 0.980.The samples could be classified into four categories according to their main origins by chemical pattern recognition,and the results of cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis were all able to discriminate the samples of different main origins effectively.The com
作者
冉淳莹
王杰
陈颖馨
马彦红
殷莉丽
王浩涵
蔡晓洋
黎智
李敏
张添植
RAN Chunying;WANG Jie;CHEN Yingxin;MA Yanhong;YIN Lili;WANG Haohan;CAI Xiaoyang;LI Zhi;LI Min;ZHANG Tianzhi(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Sichuan Tianzhi Chinese Herbal Medicine Co.Ltd.,Luzhou 646001,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2023年第22期121-127,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
四川省科技厅重点研发项目(2020YFS0303)。
关键词
枳壳
高效液相色谱法(HPLC)
特征图谱
聚类分析
主成分分析(PCA)
灰色关联度分析
化学计量学
熵权法
Aurantii Fructus
high performance liquid chromatography(HPLC)
specific chromatogram
cluster analysis
principal component analysis(PCA)
grey correlation analysis
chemometrics
entropy weight method
