摘要
目的探讨水溶性膳食纤维低聚果糖(FOS)对溃疡性结肠炎(UC)小鼠肠黏膜屏障的保护作用,为UC的治疗寻找新的药物选择。方法采用4%葡聚糖硫酸钠诱导7 d建立UC小鼠模型,将6~8周雄性C57BL/6小鼠随机分为3组:对照组饮用蒸馏水;模型组给予4%葡聚糖硫酸钠;FOS组在4%葡聚糖硫酸钠诱导的同时给予20 mg/mL的FOS灌胃;每日监测小鼠体重、粪便性状及便血情况,计算疾病活动指数(DAI)评分。实验结束后,HE染色观察小鼠结肠组织病理改变,应用实时荧光定量PCR、Western Blot及免疫荧光染色检测各组小鼠肠道中紧密连接蛋白ZO-1、Claudin-1、Occludin的表达水平。结果与对照组相比,模型组小鼠体重降低、DAI评分升高;而FOS组小鼠体重高于模型组,DAI评分低于模型组(P<0.05)。与对照组相比,模型组小鼠结肠长度缩短[(7.52±0.41)cm比(5.48±0.19)cm],组织病理学评分增高(0.53±0.38比3.51±0.18);而FOS组小鼠结肠长度[(6.82±0.63)cm]长于模型组,病理学评分(2.33±0.63)低于模型组,差异均有统计学意义(P<0.05)。模型组小鼠ZO-1、Claudin-1、Occludin蛋白及其mRNA表达水平均较对照组降低(P<0.05);而FOS组小鼠均较模型组增高(P<0.05)。结论FOS能够减轻UC小鼠体重下降症状,降低其DAI评分,改善结肠组织炎症,上调紧密连接蛋白ZO-1、Claudin-1、Occludin的表达,保护肠黏膜屏障。
Objective To investigate the protective effect of water-soluble dietary fiber fructooligosaccharides(FOS)on intestinal mucosal barrier in mice with ulcerative colitis(UC),and to find a new drug option for the treatment of patients with UC.Methods This study used 4%dextran sodium sulfate(DSS)to induce a 7-day UC mouse model.Male C57BL/6 mice aged 6-8 weeks were randomly divided into three groups:the control group drank distilled water;The model group was given 4%DSS;The oligofructose group was administered 20 mg/mL FOS by gavage simultaneously with DSS induction.The body weight,fecal characteristics,and fecal blood status of mice daily,and the disease activity index(DAI)score were monitored.After the experiment,HE staining was used to observe the pathological changes in the colon tissue of mice.Real time fluorescence quantitative PCR,Western Blot,and immunofluorescence staining were used to detect the expression levels of tight junction proteins ZO-1,Claudin-1,and Occludin in the intestines of each group of mice.Results Compared with control group,the weight of mice decreased and the DAI score increased in model group(P<0.05).However,the weight of mice in FOS group was higher than that in model group,and the DAI score was lower than that in model group(P<0.05).Compared with control group,the colon length of mice in model group was shortened[(7.52±0.41)cm vs.(5.48±0.19)cm],and the histopathological score was increased(0.53±0.38 vs.3.51±0.18).However,the colon length of mice in FOS group[(6.82±0.63)cm]was longer than that in model group,and the hispathological score(2.33±0.63)was lower than that in model group.All the differences were statistically significant(P<0.05).The expression levels of ZO-1,Claudin-1,Occludin protein and its mRNA levels in model group were lower than those in control group(P<0.05).However,those of FOS group were higher than those in model group(P<0.05).Conclusion FOS could alleviate the symptoms of weight loss in UC mice,reduce their DAI score,improve inflammation of colon tissue,upregu
作者
曹培同
吴捷
Cao Peitong;Wu Jie(Department of Pediatric Gastroenterology,Shengjing Hospital of China Medical University,Shenyang 110004,China;Department of Gastroenterology,Beijing Children′s Hospital,Capital Medical University,National Center for Children′s Health,Beijing 100045,China)
出处
《中国小儿急救医学》
CAS
2023年第10期770-775,共6页
Chinese Pediatric Emergency Medicine
关键词
溃疡性结肠炎
低聚果糖
肠黏膜屏障
紧密连接蛋白
Ulcerative colitis
Fructooligosaccharides
Intestinal mucosal barrier
Tight junction protein
