摘要
为探究大径材培育目标下间伐和施肥对杉木(Cunninghamia lanceolate)近熟林土壤微生物特征的影响,了解微生物生物量碳氮与微生物群落结构的相互关系,以福建省洋口国有林场31年生的杉木近熟林为对象,设置间伐(T)、间伐+施肥(TF)、对照(CK,不间伐不施肥)3种处理,研究不同处理下杉木近熟林土壤微生物生物量碳(SMBC)和土壤微生物生物量氮(SMBN)含量及其化学计量特征(SMBC/SMBN)、土壤微生物熵碳(qMBC)和土壤微生物熵氮(qMBN)的变化规律,采用高通量测序技术分析不同处理下土壤微生物群落结构差异。结果表明,T和TF处理能提高SMBC含量,增大qMBC和SMBC/SMBN,且TF处理的提高幅度较大;T处理会降低SMBN含量和qMBN。经T和TF处理后土壤真菌群落优势菌门的丰富度和多样性指数升高,而细菌与之相反。相关分析表明,SMBN含量、q MBN和SMBC/SMBN与土壤细菌和真菌群落结构关系密切;SMBN含量和q MBN与土壤真菌丰富度、多样性指数呈显著负相关(P<0.05)。综合认为,T和TF处理能够改善林地土壤微生物生存环境,促进真菌生长和繁殖,有利于提高土壤肥力,为杉木大径材培育提供良好的土壤条件。
In this study,we investigated the effects of thinning and fertilization on soil microbial properties in a near-mature Chinese fir(Cunninghamia lanceolate)plantation with the goal of large-diameter timber cultivation,and we determined the correlation between microbial biomass carbon and nitrogen properties and microbial community characteristics.Thus,a 31-year-old Chinese fir plantation in Yangkou state-owned forest farm in Fujian Province was chosen as the study objective,and three treatments were set:thinning(T),thinning+fertilization(TF),and control(CK,no thinning or fertilization).The soil microbial biomass carbon(SMBC),soil microbial biomass nitrogen(SMBN)contents,its stoichiometric characteristics(SMBC/SMBN),and the change in soil microbial entropy carbon(qMBC)and soil microbial entropy nitrogen(qMBN)were measured in different treatments in this study.The high-throughput sequencing technology was used to analyze changes in the soil microbial community characteristics in different treatments.The results showed that the SMBC content,qMBC,and SMBC/SMBN values increased in the T and TF treatments.In addition,the TF treatment exhibited a higher increment.Conversely,the SMBN content and qMBN decreased in the T treatment.Furthermore,the T and TF treatments displayed higher levels of richness and diversity index concerning the relative abundance of dominant phyla in the soil fungi community.However,contrasting results were observed for bacteria,as lower richness and diversity index values were observed in the T and TF treatments.Correlation analysis showed that SMBN content,qMBN,and SMBC/SMBN were closely related to bacterial and fungal community structure;SMBN content and qMBN were significantly negatively correlated with soil fungal richness and diversity index(P<0.05).Overall,the T and TF treatments improved the living environment of soil microorganisms and promoted the growth and reproduction of fungi and improve soil fertility,which provided a good soil condition for the cultivation of Chinese fir large-diamete
作者
田地
陈义堂
史月冬
连书钗
边黎明
唐罗忠
TIAN Di;CHEN Yitang;SHI Yuedong;LIAN Shuchai;BIAN Liming;TANG Luozhong(Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing,Jiangsu 210037,China;College of Forestry,Nanjing Forestry University,Nanjing,Jiangsu 210037,China;Yangkou State Forest Farm,Shunchang,Fujian 353211,China)
出处
《森林与环境学报》
CSCD
北大核心
2023年第6期569-578,共10页
Journal of Forest and Environment
基金
福建省科技计划项目“杉木大径材良种选育及高质量培育技术研究”(2022N0018)
江苏高校优势学科建设工程资助项目(PAPD)。
关键词
杉木
近熟林
间伐
施肥
土壤微生物群落
Chinese fir
near-mature forest
thinning
fertilization
soil microbial community
